شناسايي محصولات كشاورزي تراريخته با استفاده از روشهاي مبتني بر PCR

چكيده انگليسي :

PCR based Identification of Genetically Modified Crops Faezeh Fouladgar F Fouladgar@ag iut ac ir faezehfouladgar@yahoo com May 13 2015 Department of Agricultural Biotechnology Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiSupervisors M Talebi Ph D Assoc Professor mtalebi@cc iut ac ir M Bahar Ph D Professor mbahar@cc iut ac irAbstract Genetically modified GM plants are one of the great achievements of modern biotechnology in agriculture They in recent years have conquered the global food market insofar as the land under the cultivation of themand the number of farmers who cultivate these crops have been constantly increasing However there is still nota widespread use of this type of agricultural production and even the distribution of genetically modified GM crops is banned in some countries Therefore due to the global trade of GM products the customs rules of thesecountries require that imported products should be inspected for the presence of additional genes Suchinspections require the use of appropriate methods to determine the presence of GM genes in agriculturalproduction According to international biosafety law the import of genetically modified plants without thepermission of Biosafety Committee into the country is prohibited but since some products are not imported intothe country through customs ports identification between GM non GM products has a great importanceespecially in the consumer market products This study was carried out to detect genetically modified crops inthe markets of Iran using PCR based methods In this study samples of crops including tomato rice soybean corn canola and cotton were collected from various sources such as shops greenhouses livestock and poultryfactories and farms in Isfahan The initial screening of all samples was performed using primer pairs P35S F Rfor CaMV35S promoter and NOS 1 NOS 3 for NOS terminator DNA samples of tomato soybean canola cotton and corn amplified 195 bp and 180 bp a part of 35S promoter and NOS terminator sequence respectively The sequence of fragments verified by comparison with known sequences in GeneBank andindicated a 100 homology In rice samples it was not observed any band indicating the presence of NOSterminator and 35S promoter region Finally GMO specific primers were used for the selective detection andidentification of selected transgenic plants Therefore the tomato samples were tested for the presence ofantisence polygalactoronase gene by means of the primer pair PG F R which generate an amplicon 384bp in allsamples that had 35S promoter or NOS terminator band or both with 100 similarity in GeneBank databaserelated sequenced Amplification of 498 bp in cotton canola and soybean samples by means of the primer pairCP4 EPSPS F R and its high homology 99 with the sequence of 5 Enolpyruvylshikimate 3 Phosohatesynthase gene confirmed the presence of CP4 EPSPS gene in these crops In order to identify GM corns Cry1A b F R were used to detect endotoxin cry gene and all of corn samples generated an amplicon 322bp inlength by 100 similarity with sequences of Cry1A b in GeneBank database In the present study ricesamples did not generate any band using the tested primers The results of this study showed that the GM cropsare available in Iranian markets without consumers awareness Keywords Iran GM crops PCR Detection

فولادگر، فائزه

شناسايي محصولات كشاورزي تراريخته با استفاده از روشهاي مبتني بر PCR

شناسايي , ايران