بهينه سازي توليد و جداسازي كيتوزان قارچي

چكيده انگليسي :

62 Optimization of production and Separation of fungal chitosan Farideh Ezadi f ezadi@ce iut ac ir Date of submission 13 January 2016 Department of Chemical Engineering Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language PersianSupervisor Keikhosro Karimi Karimi@cc iut ac irAbstractChitosan is a copolymer containing glucosamine and N acetyl glucosamine units in whichglucosamine units are predomination Chitosan has numerous applications in biotechnology food agriculture pharmacy and medical It commercially produces by deacetylation of chitin in shells ofcrustaceans Nowadays the alternative source of chitosan is cell wall of zygomycetes fungi inwhich chitosan appears naturally Recent studies show that fungal cultivation conditions includingthe composition of culture media strongly influence the growth of zygomycetes and chitosanaccumulation in the cell wall In this study the effects of different nitrogen source urea andammonia with carbon to nitrogen ration C N of 7 5 10 and 15 were studied on chitosanproduction by Mucor indicus A full nutrient medium containing ammonium sulfate was used as abase Then other nitrogen source with three amount of C N was added to the base medium withoutammonium sulfate for various morphology aerobic filamentous aerobic yeast like and anaerobicyeast like of M indicus The concentration of sulfate ions was kept at its concentration in the basemedium by adding 5 68 grams per liter of sulfuric acid Furthemore a medium without nitrogensource was evaluated for each morphology The biomass efficiency for three nitrogen sources wasincreased more in the case of aerobic yeast like morphology The maximum biomass yield of 0 131 0 001 g g glucose was observed by adding carbon to nitrogen ratio of 15 using sodium nitrate inaerobic yeast like morphology After 48 h fermentation anaerobic yeast like morphology resultedin the highest ethanol yield where addition of urea showed the highest ethanol yield of 0 473 0 005 g g glucose at C N 7 5 On the other hand addiotion of sodium nitrate resulted in the highestchitin and chitosan yield of 0 73 g g AIM under aerobic filamentous morphology at C N 7 5 Then the maximum of glucosamine yield in the cell wall was detected for the amples that had thehighest amount of chitin and chitosan Glucosamine yield was higher in the samples prepared underaerobic filamentous morphology 0 627 0 006 g g AIM while the phosphate content was theminimum 0 540 0 004 g g AIM The minimum glucosamine yield was achieved for the aerobicyeast like morphology 0 313 0 026 g g AIM The maximum of N acetyl glucosamine yieldwas for urea nitrogen sources in aerobic filamentous morphology with C N 15 0 116 0 001 g gAIM Chitosan is derived from the best samples that had the highest efficiency of chitin andchitosan The highest chitosan extraction efficiency was achieved for sodium nitrate as nitrogensource under the aerobic filamentous morphology in fermentation 14 1 g g AIM Keywords Chitosan chitin nitrogen source morphologhy culture media

ايزدي، فريده

بهينه سازي توليد و جداسازي كيتوزان قارچي

منبع نيتروژن , مورفولوژي , موكوراينديكوس , محيط كشت