پديد آورنده :
سعادت نيا، روح انگيز
عنوان :
توليدكالوس و جوانه زني رويان بالغ در زالزالك(.Crataegus Sp. L)
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
علوم باغباني
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
هشت، ۳۸ص.: مصور، جدول، نمودار
استاد راهنما :
بهرام باني نسب ، سيروس قبادي
استاد مشاور :
بدرالدين ابراهيم سيدطباطبايي
توصيفگر ها :
زالزالك , جنين بالغ , باززايي , ريزازديادي , كالوس
استاد داور :
مهديه غلامي، مجيد طالبي
تاريخ ورود اطلاعات :
1396/09/01
چكيده انگليسي :
39 Callus Induction and Plant Regeneration from Mature Embryo of Seeds of Two Hawthorn Crataegus Sp L Species Rohangiz Saadatnia r saadatnia@ag iut ac ir June 21 2017 Department of Horticultural Science College of Agriculture Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiSupervisors Bahram Baninasab bbanin@cc iut ac ir Cyrus Ghobadi cyrus@cc iut ac irAbstractHawthorn Crataegus sp is a large genus of shrubs and trees in Rosaceae which is used as ironchlorosis and drought tolerant rootstocks planting in urban green space and production of medicinalherbs The challenges seedling manufacturers facing is double dormancy and decrease in germination which it takes 2 3 years time to produce Hawthorn seedling Tissue culture and micropropagationtechniques could be used for fast proliferation of this plant This study was conducted as a completelyrandomized factorial design We used mature embryo of seeds as explants in sterile conditions underlaminar airflow cabinet that was separated from the endosperm The first in vitro experiment was theeffect of different concentrations of 2 4 D 0 2 and 4 mgl 1 and NAA 0 1 and 2 mgl 1 hormones oncallus production in two species C atrosanguinea and C meyeri with 3 replications The second in vitroexperiment was the effect of different concentrations of Kin 0 1 and 2 mgl 1 and BAP 0 2 mgl 1 hormones on plant regeneration of mature embryo with 3 replications Callus size and callus fresh weightwere measured Result of the first expeviment showed that treated explant with 4 mgl 1 2 4 D 1 mgl 1NAA increase fresh weight and callus size in both species Unfortunately it was not possible to doexperimental analysis in the second exeperiment due to high contamination However we found thatcharcoal active decrease the amount of contamination and cold treatment did not have any effect onembryo regeneration Keywords hawthorn mature embryo micropropagation regeneration callus
استاد راهنما :
بهرام باني نسب ، سيروس قبادي
استاد مشاور :
بدرالدين ابراهيم سيدطباطبايي
استاد داور :
مهديه غلامي، مجيد طالبي
