13304

12128

كارشناسي ارشد

حشره شناسي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

۱۳۹۶

دوازده، ۸۱ص.:‌ مصور، جدول، نمودار

جهانگير خواجه علي، محمدرضا نعمت‌اللهي

قاسم عسكري سريزدي

نفيسه پورجواد، حسن كريم مجني

1396/12/08

كتابنامه

كشاورزي

مهندسي كشاورزي

ID12128

82 Evaluation of imidacloprid resistance in the populations of whitefly Bemisia tabaci Hem Aleyrodidae Fatemeh Salehi Sedeh Fateme salehi@ag iut ac ir January 14 2018 Department of Plant Protection Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiSupervisor Dr Jahangir Khajehali Khajeali@cc iut ac irAbstractCotton whitefly Bemisia tabaci is an important polyphagous insect pest which has developed resistanceto various insecticides worldwide Neonicotinoid pesticides such as imidacloprid and thiacloprid areagonists of nicotinic acetylcholine receptors nAChR Six B tabaci populations were collected fromdifferent regions of Iran Using a phylogenetic tree constructed based on the CO sequence the biotype ofMinab population was determined as B biotype Bioassays were performed using a leaf deep method andsusceptibility of the populations to imidacloprid were assayed The lethal concentration 50 values LC 50 for different populations showed diffrences in the susceptibility of B tabaci to imidacloprid 24 4 to189 8 mg L Ahvaz population with the lowest LC50 value 24 4 mg L was considered as the susceptiblepopulation LC50 values of Isfahan Kashan Gorgan and Minab populations were estimated as 136 91 106 95 141 09 and 68 31 mg L The resistance ratio of the populations ranged from 2 8 to 7 8 forimidacloprid The synergistic effects of piperonylbutoxide PBO triphenyl phosphate TPP and diethylmaleate DEM were evaluated on the susceptible Ahvaz and resistant Kashan populations to determinthe involvement of cytochrome P450 dependent monooxygenase carboxylesterase and glutathione S transferases respectively in the resistance development Based on the results PBO and TPP showed thehighest synergistic ratios of 2 and 2 4 in the resistance population of Kashan respectively The activity ofcarboxylesterases glutathione S transferases and cytochrome P450s were also measured biochemically in order to determine which enzymes may be involved in the neonicotinoid resistance There was anincrease in the activity of cytochrome P450s up to 3 fold in the resistant population Possibly cytochromeP450 dependent monooxygenases and carboxylesterases are the enzyme systems responsible forneonicotinoid resistance in the tested populations of B tabaci Keywords Bemisia tabaci resistance of imidacloprid Synergistic ratio Enzymes assay

جهانگير خواجه علي، محمدرضا نعمت‌اللهي

قاسم عسكري سريزدي

نفيسه پورجواد، حسن كريم مجني