15547

1571 دكتري

دكتري

مهندسي مواد و متالورژي

اصفهان : دانشگاه صنعتي اصفهان

1398

هفت، 119ص. : مصور، جدول، نمودار

كيوان رئيسي، فتح اله كريم زاده

مهشيد خرازيها، حجت اله ولي، سارا مهشيد

اميرحسين نوارچيان،‌ محمدعلي گلعذار، شيدا لباف

1399/02/26

كتابنامه

مهندسي مواد و متالورژي

مهندسي مواد

1399/02/27

2611581

Design and fabrication of hierarchical gold nano microelectrodes for electrochemical detection of myocardial infarction and bacteria Alireza Sanati Sichani alireza sanati@ma iut ac ir March 4 2020 Department of Materials Engineering Isfahan University of technology Isfahan 8415683111 Iran1st supervisor Keyvan Raeissi1 k raeissi@cc iut ac ir 2nd supervisor Fathallah Karimzadeh2 karimzadeh f@cc iut ac ir 1st Advisor Mahshid Kharaziha32nd Advisor Hojatollah Vali43rd Advisor Sara Mahshid5Department Graduate Program Coordinator Mahshid Kharaziha3AbstractIn this research the performance of hierarchical gold nano micro electrodes GNMEs on asuitable substrate and using an appropriate sensing strategy for detecting myocardialinfarction and bacteria is considered In this regard indium tin oxide ITO substrate wascoated by a thin layer of electrochemically reduced graphene oxide ERGO to provide auniform distribution of electrodeposited GNMEs Then in order to create a biomimeticmolecularly imprinted polymer MIP receptor a thin layer 5 5 nm of o phenylenediamine o PD in the presence of heart fatty acid binding protein H FABP was electropolymerizedon the surface of GNMEs After removal of H FABP from the polymeric matrix the MIPlayer was reached for H FABP detection Transmission electron microscopy TEM scanning electron microscopy SEM and atomic force microscopy AFM were used forcharacterization of this structure Electrochemical investigations using differential pulsevoltammetry DPV revealed a very low detection limit 0 0034 fg mL 1 and a wide linearrange of detection 1 fg mL 1 to 100 ng mL 1 due to the excellent performance of GNMEs High stability of the fabricated sensor at room temperature after three weeks owing to theapplied biomimetic receptor and very good sensory behavior resulted from GNMEs weresome advantages of this work against previous studies Thereafter GNMEs wereelectrodeposited by two stages method on a gold coated glass substrate and used for thedetection of Escherichia coli E coli The results of Electrochemical impedancespectroscopy EIS in phosphate buffered saline PBS showed a detection limit of 90 CFU colony forming unit per 30 L solution Moreover optical fluorescence microscopy imagesrevealed the sharp edges of the GNMEs were removed after the second electrodepositionstage providing a suitable hierarchical structure for capturing bacteria The results of thisPh D thesis revealed the excellent performance of GNMEs with the hierarchical structure tomake a biomimetic sensor for early detection of myocardial infarction and a multi purposesensor for detection of bacteria Keywords electrochemical sensor gold nano micro electrodes GNMEs biomimetic receptor myocardial infarction bacteria 1 Professor Department of Materials Engineering Isfahan University of Technology Isfahan Iran2 Professor Department of Materials Engineering Isfahan University of Technology Isfahan Iran3 Associate Professor Department of Materials Engineering Isfahan University of Technology Isfahan Iran4 Professor Department of Anatomy and Cell Biology McGill University Montreal Canada5 Assistant Professor Department of Bioengineering McGill University Montreal Canada 1

كيوان رئيسي، فتح اله كريم زاده

مهشيد خرازيها، حجت اله ولي، سارا مهشيد

اميرحسين نوارچيان،‌ محمدعلي گلعذار، شيدا لباف