15563

13921

كارشناسي ارشد

مرتعداري

اصفهان : دانشگاه صنعتي اصفهان

1398

دوزاده،75 ص.: مصور، جدول، نمودار

محمد رضا وهابي، پوراندخت گلكار

حسين بشري، محمد رضا سبزعليان

1399/03/20

كتابنامه

مهندسي منابع طبيعي

مهندسي منابع طبيعي

1399/03/20

2604305

The effect of salinty stress on some of the secondary metabolites of Nigella sativa L under in vitro condition Golab Bakhshi g bakhshi73@gmail com Jaunary 26 2020 Departement of Natural Resources Isfahan University of Technoligy Isfahan 84156 83111 IranDegree MSc Language PersianDr M R Vahabi vahabi@iut ac irDr P Golkar golkar@iut ac ir Abstract Soil salinity is one of the growing issues in the world which covers a large part of our country Evaluation of salinity tolerance of medicinal plants for cultivation in saline areas is essential Thisstudy was conducted to optimize callus production and then investigate the response of differentgenotypes of Nigella sativa to salt stress under in vitro culture For this aim the reaction of 10different genotypes of Nigella sativa at different levels of salinity stress 84 and 250mM wasstudied Results of variance analysis showed that the variables of the hormone genotype andmicro sample had a significant effect on callus formation percentage and growth rate The resultsof interaction between growth composition and genotypes showed that Nig2 genotype belonged toFereidoonshahr had the maximum growth rate 0 52 mm day while Nig59 genotype from Syriahad the lowest growth rate 0 18 mm day at growth composition of 3 mg L Kin and 0 5 mg LNAA compared with other genotypes Also in growth composition of 3 mg L BAP and 1 mg LNAA Bap genotype Nig1 belonged to Kerman province and Nig68 from Turkey showed thehighest and lowest callus growth rate respectively The comparison of mean interaction effectsbetween genotype and micro sample showed that the Nig2 genotype had the highest callus growthrate 0 55 mm day in the leaf sample and the Nig78 genotype had the highest callus growth rate 0 35 mm day in hypocotyl sample Comparison of mea interaction effects of growth compositionand micro sample showed that the highest growth rate of callus 0 39 mm day belonged to leafmicro sample in growth composition of 3 mg L BAP and 1 mg L NAA Thus it gives the leafmicro sample as the best known micro sample In the second part of the study differentconcentrations of sodium chloride salt 84 250mM and control were evaluated to examine theresponse of genotypes to salinity stress The results of this study showed that with increasingsalinity sodium ion content total phenol content flavonoid flavonol anthocyanin and antioxidantactivity by DPPH method and total thymol content increased significantly Potassium ion contentincreased with salinity concentration Nig1 and Nig2 genotypes were identified as in vitro salt tolerant genotypes Kywords Callus growth rate Antioxidant Activity Cell cultuer Total phenol content

محمد رضا وهابي، پوراندخت گلكار

حسين بشري، محمد رضا سبزعليان