In this research, Sodium alginate- Pectin composite (ALG-PEC CS) and NC with 0.5, 1, and 2 wt% TiO2 NPs were prepared. Using CaCl2 and glutaraldehyde (Glu) were used as cross-linkers in two separate steps. The cross-linking procedure produces rigid and stable scaffolds as the particular sites for sedimentation of hydroxyapatite (HA) and increases structural strength, it can also decrease solubility of products in water and simulated body fluid (SBF) solution to 10% or less. Moreover, increasing the amount of water and SBF absorption increases the porosity and pores that supply nutrients within the inner surfaces ALG-PEC CS and ALG-PEC/TiO2 NC as well as increase the surface area to allow more space for HA to form on the scaffold. All prepared samples were characterized via Field-emission scanning electron microscopy (FE-SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction, Ultraviolet-Visible, and Energy-dispersive X-ray spectroscopy (EDS). Then, the bioactivity and antibacterial properties of the fabricated samples were investigated. Bioactivity test was confirmed via the formation of HA on the surface of the synthesized CS and NC films. Also, the plate count method was utilized to eva‎luate the antibacterial activity of films against Staphylococcus aureus (S. aureus) bacteria. Based on comparing FE-SEM, FT-IR, and EDS results, before and after SBF exposure of the CS and NC films, it was revealed that the film containing 2 wt% TiO2 NPs had the best bioactivity performance and it had the best antibacterial activity such a way that after 1 hour all the bacteria were killed.

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