Humans always use different methods and combinations to heal wounds. Today, hydrogel dressings that combine with bioactive substances; have found significant performance in wound dressings. The excellent properties of hydrogels such as: ability to absorb high exudate, oxygen permeability, biocompatibility, ability to load and release biologically active factors have been considered.in this study sterile cotton gauze was used. Due to the reported antibacterial and antioxidant properties of honey as well as the sensitivity to pHof red cabbage extract, these two substances was placed in alginate hydrogel. Mechanical properties, FTIR spectrum, degradation rate, antibacterial properties, antioxidant percentage, pHsensitivity and cytotoxicity test of the samples were eva‎luated. The results showed that the use of honey and red cabbage extract is effective on the properties of alginate hydrogels. According to the obtained data; rupture elongation of the hydrogel was found to be 0.8 mm, the percentage of degradability in distilled water and PBS wer 93% and 83% respectively and the percentage of time dependent antioxidant property after 24 hours was 95%. These numbers are perfectly within the acceptable range for wound dressing. The results of the FTIR spectrum showed that honey has established a good hydrogen bond with alginate and also red cabbage extract has strengthened the hydrogen bond. The results of an antibacterial test showed that the hydrogel sample coated with honey was resistant to Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The color of hydrogel was different in buffers with pH9, 7 and pH= 4. The results of cell culture showed that more proliferation of fibroblasts on the hydrogel sample containing honey and red cabbage than that on the hydrogel sample alone, which is due to the simultaneous effect of honey and red cabbage on these cells. The wound dressing fabricated during this study can be used successfully to dress and heal wounds with high exudate and prevent bacterial infection until the honey is completely diluted the infection in the wound environment can also be distinguished due to variouse color of red cabbage extract at different pHs.

لاله قاسمي مياركه

فرزانه علي حسيني، حسين توانايي