In this Thesis, an electrochemical sensor was fabricated with using surface modification based on mesoporus Copper Metal-Organic Framework (Cu-MOF/MC) on glassy carbon electerode for determination of morphine. The electrochemical behavior of the electrode for the detection of morphine in phosphate buffer was eva‎luated by electrochemical methods of Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV). Optimization of effective parameters such as weight ratio of catalyst components, pH adjustment of solutions containing analyte, duration of potential application in solution, electrode retention time in solution and other parameters that affected the performance of the electrode were performed. The above electrode provides the response in a wide linear concentration range from 2.0 × 10-8-1.1 ×10-4 M. The detection limit of the proposed method was reported to be 1×10-9 M. Recovery percentages for analyte measurement in three different human plasma samples with concentrations of 2, 5 and 2 μM were 100.4%, 97.8% and 96.5%, respectively. As well, for three different urine samples with concentrations 1.54, 2.09 and 2.09 micromolar were reported 98.7%, 106.5% and 96.8%, respectively. The advantages of the built-in sensor include optimal sensitivity and selectivity, low detection limit, short response time and simplicity of the equipment used.

بهزاد رضايي

علي اصغر انصافي

كيومرث زرگوش، اسماعيل حيدري