Abstract Sansevieriais native to West Africa and South Asia and has more than 70 species that grow in relatively hot and dry areas. It is a monocot and perennial plant with various shapes and colors with ornamental leaves and a relatively high economic value. Sansevieriais an ornamental plant that is also used for medicinal and industrial purposes. The leaves of this plant are ornamental and the plants of this genus have various shapes and colors. Sansevieria trifasciata "Laurentii" is very important in terms of ornamentation, marketability and economy. Propagation of this plant through cuttings mainly produces non-stemming and green plants. In this research, the effect of culture medium and explants on the asexual reproduction of Sansevieria trifasciata "Laurentii" and also the possibility of transferring the trait of golden leaf margins through tissue culture were investigated. In this research, three main objectives were investigated in general. 1- The effect of different concentration of growth regulator on the rate of callus production, branching and rooting. 2- The effect of the type of explant on the mentioned traits and also the production of chimeras. 3- The effect of seedling height on rooting traits. After disinfecting the leaves, explants were prepared from three parts of the leaves separately (the golden part in pure form, the chimeras part (green and golden together) and the green part). In order to induce callus, explants were transferred to MS culture medium containing growth regulator 2,4-D with different concentrations and at the end of callus formation, traits such as callus formation percentage, weight and volume of callus and their quality was investigated and the best explant was green tissue and treated with 2,4-D with a concentration of 0.45 mg/l. To induce branching and seedling formation, the produced calli were transferred to MS culture medium containing BA hormone with different concentrations, which were eva‎luated by measuring the characteristics (number of seedlings and asexual embryos produced, seedling height, regeneration percentage) branches and number of leaves) in this stage, the green tissue explant had the highest amount of production in terms of the number of branches and the production of asexual embryos. For the rooting stage, the resulting seedlings that had reached the appropriate height and growth (the ones that had formed leaves and fully opened and had a height of at least 1 cm) were added to 1.2MS culture medium containing IBA hormone with a concentration of 2mg/ l were transferred and traits such as rooting percentage, number and length of roots were measured. Plants whose length was 2 cm at the time of transfer had the best results. Five completely golden seedlings were also obtained, which did not produce any roots in the rooting medium. When the size of the roots reached 5 cm, they were planted in small pots and transferred to the greenhouse after adaptation.

نعمت اله اعتمادي، مهدي رحيم ملك

مهديه غلامي، مجيد طالبي