In this research, 14 adult stallions with similar characteristics and with an average weight of 400±30 kg were selected and randomly divided into 2 groups of 7 horses. A completely randomized design was employed for 60 days. The experiment trail carred on two treatments including control (basal ratio) and experimental group (basal ratio +1000 mg per hundred kilograms of body weight, a mixture of trace minerals resources of iron, copper, cobalt, manganese, zinc, cream, selenium, magnesium per day) at the disposal of horses. The ration was adjusted based on the nutritional requirements and given to the horses in five times at 7, 10, 14, 17 and 21 hours. In order to increase the accuracy of experiment the mixture of trace elements were weighted daily and mixed well with the concentrate by specified measures and then given to the horses. In order to have the same exercise activity and check the physiological responses, the horses were trained daily. The training steps included 10 minutes of walking, 15 minutes of trotting and four short hoofs, and 5 minutes of marching. After the 7-day adaptation period, on 30th and 60th days of the experiment before and immediately after certain sports activities, 20 ml of blood samples were taken from the Vadaj vein and blood factors (glucose, total antioxidant capacity, cholesterol, triglyceride, urea) were investigated and eva‎luated. Also, the activity of liver health indices (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyl transferase) were eva‎luated on the 30th and 60th days of the experimental trial before and after of sport activities. In these days, the differential white blood cells count as well as heart and breathing rates were eva‎luated. Our results showed that on 30th day of the experiment, before the exercise only the concentration of cholesterol decreased marginally (P=0.09). After exercise on this day, total antioxidant capacity was maintained at higher levels (P=0.59). The results of blood biochemical parameters on 60th day of the experiment show that before exercise, urea concentration decreased (P<0.01). In the same day, after exercise, glucose concentration and total antioxidant capacity increased and urea concentration decreased (P<0.01). At the end of the experiment, the enzyme activity of alkaline phosphatase (P<0.01) and gamma glutamyl transferase (P<0.05) were lower in experimental treatment. The profile of white blood cells was not affected by treatment, but the ratio of neutrophils to lymphocytes was lower on day 60 (P=0.07). The experimental treatment did not make a significant difference in terms of heart and breathing rate. Accordingly, the findings of the present experiment indicated that, the trace minerals can improve the metabolic and antioxidant indices of the blood and improve the intensity of movement stress via improving the hepatic enzyme activity and the profile of white blood cells.

اميرحسين مهدوي

سميه مجاهدي

فرزاد هاشمزاده , نفيسه سلطاني زاده