Human amniotic membrane is a multilayered membrane rich in hyaluronic acid, types of collagen, laminin, fibronectin, elastin and proteoglycans, which can act as an extracellular matrix. Amniotic is mainly used for dressing burn wounds and tissue engineering. In order to reduce the cost and ease of application of amniotic membrane for dressing, it is combined with biocompatible polymers to simulate the extracellular matrix of the skin. In this research, a suspension of decellularized human amniotic membrane was prepared from the combination of formic acid/acetic acid solvents for use in polyhydroxybutyrate polymer solution. In the next step, electrospun scaffolds were prepared from polyhydroxybutyrate and amniotic polymer with different ratios. In order to improve the mechanical properties of the scaffold, the mixing of polyhydroxybutyrate and polycaprolactone polymers with different volume ratios was considered. Concentrations of 5% and 10% w/w based on polymer weight were prepared. For further comparison, polycaprolactone and polycaprolactone/amniotic5% w/w scaffolds were spun as control scaffolds. After electrospinning, the morphology of the scaffolds was checked using field emission scanning electron microscope (FE-SEM) images, and by increasing the concentration of polycaprolactone, smooth nanofibers without beads were obtained, and by adding amniotic to the scaffolds, the diameter of the nanofibers decreased from 600 nm to 143 nm. The mechanical properties of the scaffolds were investigated and the highest strength was measured for the composite scaffolds of polyhydroxybutyrate/polycaprolactone/amniotic 10% w/w with a strength of 2.46 MPa and polycaprolactone/amniotic 5% w/w with a strength of2.79 MPa. After checking the hydrophilicity, the contact angle and wetting time for the optimal scaffold were 42 degrees and 15 seconds, respectively. To eva‎luate cell growth and adhesion, mesenchymal cell culture on electrospun scaffolds was used using field emission scanning electron microscope images from the first and third day of culture. The best cell culture results were related to polyhydroxybutyrate/polycaprolactone/amniotic 10% w/w scaffold. The antibacterial property of the scaffolds was investigated using the non-growth halo method with Staphylococcus aureus and Escherichia coli bacteria, and the polyhydroxybutyrate/polycaprolactone/amniotic 10% w/w scaffold had antibacterial properties.

فرزانه علي حسيني

لاله قاسمي مباركه , حسين فشندي