The present study was designed to eva‎luate the effects of adding lysophospholipid (LPL), specifically Lipidol, to diets with different energy levels on the performance, egg quality, hatchability, and chick quality of broiler breeder hens in two experiments. In the first experiment, a total of 24 roosters and 240 Ross 308 broiler breeder hens were used in a 2×2 factorial arrangement (two levels of energy: 2800 and 2760 kcal/kg, and two levels of LPL: 0 and 500 g/ton) with four treatments and six replicates, with each replicate consisting of 10 hens and one rooster for a period of 12 weeks. The diets used in this study were as follows: 1) standard diet of corn and soybean (energy: 2800 kcal/kg), 2) standard diet of corn and soybean + 500 g/ton LPL, 3) diluted diet (energy: 2760 kcal/kg), and 4) diluted diet + 500 g/ton LPL. Performance parameters including egg production percentage, daily and weekly hatchability percentage were eva‎luated, and the egg production percentage of broiler breeder hens was significantly improved (p<0.05) by the addition of LPL to the diets. Furthermore, egg quality parameters were eva‎luated at weeks 4, 8, and 12, and LPL resulted in an increase in yolk color of the eggs (p<0.05). Blood biochemical parameters were also assessed by blood sampling from broiler breeder hens, and the levels of triglycerides were reduced in the LPL treatment group at week 8, while they were increased at week 12. Moreover, the enzyme AST was improved at week 8, and the enzyme ALT was improved at week 12 (p<0.05). At the end of the experiment, five broiler breeder hens from each treatment were slaughtered, and it was shown that the weight of the liver and abdominal fat of hens fed with LPL was significantly lower than those fed with the control diet (p<0.05). In the diet group with reduced energy level, TGF-β gene expression was significantly higher than other groups (P<0.05). Diet with reduced energy and diet with reduced energy level + LPL significantly reduced the amount of PGC-1α gene transcription (P<0.05). In the second experiment, a total of 640 one-day-old broiler chicks were used, which were born from broiler breeder hens aged 57 and 61 weeks and were fed with the experimental diets for 8 and 12 weeks, respectively. The experimental design was a 2×2 factorial arrangement with four treatments, seven replicates, and 10 birds per replicate for the first batch, and four treatments, nine replicates, and 10 birds per replicate for the second batch. The diets in all four treatments were similar and based on the control diet. The results showed that adding LPL to the diets of broiler breeder hens led to a significant improvement (p<0.01) in the average body weight (ABW), average daily weight gain (ADG), and feed conversion ratio (FCR) of 7-day-old chicks hatched from hens that were fed with LPL for 8 and 12 weeks. The comparison of main effects showed that feeding broiler breeder hens with the control diet resulted in an increase in the body weight of the produced chicks at the end of the first week of rearing. Additionally, the serum cholesterol level of the hatched broiler chicks from hens fed with the control diet was increased (p<0.05). Furthermore, feeding broiler breeder hens with the LPL diet for 12 weeks resulted in a decrease in serum triglyceride levels (TG) and aspartate transaminase enzyme (AST) activity in the hatched chicks (p<0.01). Moreover, adding LPL to the diets of broiler breeder hens for 12 weeks led to a decrease in the activity of hepatic superoxide dismutase enzyme (SOD) (p<0.01) and an increase in the activity of hepatic glutathione peroxidase enzyme (Gpx) (p<0.05) in the newly hatched broiler chicks. The intractionshowed that effects, lipoprotein lipase, LPCAT3 and CPT1A genes, which are involved in the mechanism of hepatic β-oxidation, were significantly increased in the liver tissue of offspring with LPL consumption (P<0.05).

محمد صدقي

انور آموزمهر

اميرحسين مهدوي , نفيسه سلطاني زاده