Abstract Periodontitis is a major public health problem. If left untreated, periodontitis may affect the pathogenesis of several systemic diseases such as diabetes, cardiovascular diseases, and adverse pregnancy outcomes. Periodontitis or gum disease is the sixth most common disease worldwide that damages the soft tissue and supporting bone of the tooth and the main cause of tooth loss is considered to affect health and quality of life. Periodontitis leads to progressive destruction of periodontal tissues including alveolar bone, periodontal ligament and connective tissues. The conventional treatment of this disease mainly includes root scaling and exfoliation to reduce the bacterial load, but the regeneration of the destroyed tissues is the ultimate goal of periodontal treatment because it has been shown to improve the function and long-term preservation of the tooth. The aim of this study is to fabricate and characterize polyhydroxybutyrate/gelatin nanofibrous membrane containing bridigiite nanoparticles for alveolar bone regeneration. In the field of periodontal and bone regeneration, artificial membranes must combine both mechanical and biological properties to prevent their collapse inside the defect. To achieve this goal, electrospinning technique has been used to synthesize membranes. Nanofibrous layers were prepared in three different ways with a concentration of 20% by weight/volume and were eva‎luated. Based on the results, the increase in bridgeite nanoparticles has decreased the diameter of the fibers and increased the specific surface area and the contact surface, as well as increased the connection between the fibers. For this reason, there is a possibility of increasing strength. The contact angle for all three samples of polyhydroxybutyrate and polyhydroxybutyrate/gelatin and polyhydroxybutyrate/gelatin/bridgegit nanofibers is 19, 24, and 25 respectively, which shows that the presence of bridgegit nanoparticles increases the hydrophilic properties. Samples 1-3 (except sample 1 at a concentration of 1000 μg/ml in a period of 96 hours) at concentrations of 10-1000 μg/ml after a period of 24, 48, 96 hours are not toxic on MG63 cells and the results of toxicity are acceptable for bone regeneration applications.

افسانه ولي پوري , فرزانه علي حسيني

منيره كوهي

داريوش سمناني , حسين فشندي