Artificial insemination in goats is an effective method for breeding and reproductive management. One of the important and vital stages of artificial insemination is the protection of goat sperm during the storage process in liquid or frozen form. The fertilization of a large number of goats with the sperm of superior goats, as well as the need to use male goats during the reproductive period or at different times of the year, stimulated more research on sperm storage. The sperm storage process causes severe damage to the sperm cell. Also, in the process of sperm freezing or cold storage, in addition to being exposed to cold or osmotic shock, these cells are also severely subjected to oxidative stress. Antioxidants are the primary defense against this oxidative damage. The bioactive compound berberine is an alkaloid that is extracted from barberry, turmeric and ergon grape and has a very strong antioxidant property. Considering the adverse effects of free radicals in oxidative stress reactions, the presence of antioxidant compounds that are able to protect sperm from damage caused by oxidative stress is essential. On the other hand, the main limitation of this antioxidant is its very low absorption rate. Accordingly, in order to use this valuable material from the nanotechnology technique using the green synthesis method and using plant extract as well as the conjugation method of this material on the transporter called selenium nanoparticles synthesized in the green method, which is a safe substance for cells. is used, because this method of nanoparticle synthesis is a completely safe and economically viable method compared to other nanoparticle synthesis methods. Also, the synthesis of nanoparticles was confirmed by EDX, UV-Visible, FE-SEM, Zeta-Potential and FTIR tests. Therefore, the purpose of this experiment is to investigate the effect of nanoberberine and selenium nanoparticle synthesized by green conjugated method with berberine on the parameters of goat sperm in cold and frozen storage conditions. In this study, after measuring the concentration of sperm parameters, the production of reactive oxygen species (ROS), fertility ability, and the development ability of eggs inseminated with treated sperm were eva‎luated in different groups. The results obtained from this study showed that all the treatments were significantly different in terms of motility, viability, membrane integrity, ROS level, lipid peroxidation, as well as sperm cell DNA health, fertility ability, and the development ability of inseminated eggs with sperms treated with the control group. and berberine treatment conjugated to green selenium nanoparticle and the simultaneous use of selenium nanoparticle and berberine nanoparticle showed the best results and showed a significant difference with other treatments. After conducting concentration measurements, eight treatments were selected, including the control group (without any additives), Br10, Br-NPs0.1, Se100, Se-NPs1, Se100+ Br10, Br-NPs0.1+ Se-NPs1, and 0.01Br-co-Se-NPs, and sperm-related parameters including motility, viability, membrane integrity, production of reactive oxygen species (ROS), lipid peroxidation, DNA damage, and finally the fertility and developmental potential of eggs inseminated with treated sperm in different groups were eva‎luated. The results showed that all treatments compared to the control group increased the motility, viability and sperm with healthy membranes in both freezing and cold storage methods, and the 0.01Br-co-Se-NPs treatment showed better results than all treatments in all three parameters (P < 0.05).

اميرحسين مهدوي , مهدي حاجيان

ليلا سلطاني , محمد حسين نصر اصفهاني

آرمين توحيدي , مهدي خدايي مطلق , رسول كوثر