ريز استخراج پراكنده فاز جامد مغناطيسي بر اساس جاذب Fe3O4 داراي عامل آمين براي استخراج كلوميپرامين از بافت پلاسما و ادرار انسان قبل از اندازه گيري توسط طيف سنج تحرك يوني

Amino-functionalized Fe3O4 nanoparticles (NPs) were developed as an effective adsorbent for the extraction an‎d determination of clomipramine (CLP) in plasma an‎d urine samples using ultrasoundassisted dispersive magnetic solid-phase microextraction (UA-DMSPE) coupled with ion mobility spectrometry (IMS). Magnetic Fe3O4 nanoparticles were synthesized via a chemical co-precipitation method. The preparation of amino-functionalized Fe3O4@SiO2-NH2 nanoparticles was confirmed by Fourier-transform infrared spectroscopy (FT-IR), energy-dispersive X-ray spectroscopy (EDX), an‎d scanning electron microscopy (SEM). The extraction method comprised two main steps: first, adsorption of the analytes on to the surface of the magnetic nanoparticles from the sample matrix: second, desorption of the adsorbed analytes from the adsorbent surface using an appropriate elution solvent. To achieve high extraction efficiency, the effects of various parameters including types an‎d volumes of extraction an‎d dispersive solvents, solution pH, an‎d extraction time were investigated an‎d optimized. Under optimized conditions, extraction was performed using 10 mL of the extraction solvent an‎d 200 µL of methanol as the dispersive solvent at pH 9 an‎d room temperature for 5 minutes. The extracted sample was subsequently injected into the IMS device (CD-IMS) for analysis. Analytical figures of merit included a limit of detection (LOD) of 20 µg/L, a linear range of 30 to 1500 µg/L with a correlation coefficient (R²) of 0.998, an‎d relative stan‎dard deviations (RSD) of 5% for three replicate extractions within one day an‎d 6% for three replicates over three consecutive days. Real urine an‎d plasma samples were selec‎ted as authentic biological matrices to eva‎luate the applicability an‎d efficiency of the developed method

محمد تقي جعفري

محمد سراجي , نفيسه فهيمي كاشاني