Abstract In this study, the imine ligan‎d (E)-2-((anthracen-9-ylmethylene)amino)-5-methylaniline an‎d then copper(II), nickel(II) an‎d zinc(II) complexes with formulas 𝐶𝑢(𝐶, 𝑁, 𝑁)𝐶15𝐻9𝑁𝐶6𝐻4 𝑁𝐻2𝐶𝐻3 (𝑁𝑂3)2 ] ,[𝑍𝑛(𝐶, 𝑁, 𝑁)𝐶15𝐻9𝑁𝐶6𝐻4𝑁𝐻2𝐶𝐻3 (𝑁𝑂 3)2 ],[𝑁𝑖(𝐶, 𝑁, 𝑁)𝐶15𝐻9 𝑁𝐶6𝐻4𝑁𝐻2𝐶𝐻3(𝑁𝑂3)2 ] were synthesized. Fourier-transform infrared (FT-IR), 1H-NMR an‎d 13C-NMR spectroscopy techniques were used to study the structural characterization. In the second stage of the research, their interaction with biological macromolecules was investigated using spectroscopic methods including ultraviolet-visible absorption an‎d fluorescence emission, which resulted in the demonstration of the interaction of the compounds with the major grooves of deoxyribonucleic acid an‎d different sites of bovine serum albumin. The data obtained from emission spectroscopy indicated that the mechanism of quenching of albumin emission by the synthesized compounds is static. Competitive titration with warfarin, digoxin an‎d ibuprofen as markers present in bovine serum albumin an‎d synthesized compounds was also performed. Also, cytotoxicity test on breast cancer cell line showed relatively good activity of prepared compounds in inhibiting cancer cells.

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فاطمه داور , غلامحسين محمدنژاد شيرازي