بررسي پتانسيل كمپلكس ليپوپپتيد باكتريائي-كلسيم براي توليد آدامس غني شده و دوباره معدني شدن دندان

Micronutrient deficiencies, especially minerals, are one of the major public health challenges in the world, with a higher preva‎lence in low-income countries. Calcium plays a key role in maintaining the structure an‎d strength of hard tissues, especially teeth, an‎d its deficiency can lead to weakening of tooth enamel, reduced mineral content of dentin, an‎d problems such as dentin hypersensitivity. In recent years, approaches based on dentin remineralization have been considered as the most effective solution to reduce this condition. In the present study, calcium-containing complexes were prepared an‎d their potential for tooth remineralization an‎d the production of calcium-enriched chewing gum was investigated. Calcium carbonate was used to provide the calcium component of the complex, an‎d microbial lipopeptides were used as their carriers to increase its bioavailability. Previous studies have shown that the calcium in this complex has high bioavailability. In this project, the effect of these complexes on decayed dentin was investigated to determine the potential of this material for use in tooth remineralization. In the next phase of the project, the enrichment of gum using calcium complexes was carried out an‎d the properties of the gum were investigated. For this purpose, human molars were collected from medical centers an‎d their dentin part was separated during cutting. Healthy dentin was decayed using citric acid solution an‎d their decay was confirmed using a Quanta 450 FEG field emission scanning electron microscope. Decayed teeth were treated with calcium complexes an‎d compared with teeth treated with artificial saliva an‎d calcium carbonate salt (as a control). The results of this study showed that teeth treated with calcium complexes were significantly remineralized, which was confirmed by analyses of hardness, roughness, porosity, crystallinity, an‎d tooth morphology. This was while teeth treated with artificial saliva were more decayed an‎d the remineralization rate of teeth treated with calcium carbonate salt was significantly lower than that of teeth treated with complexes. In the second phase of the study, the results showed that this enrichment did not cause significant changes in the texture, ash percentage, an‎d sensory properties of the gum, an‎d only a significant difference was reported in the color of the enriched samples. In the continuation of this project, the rate of calcium release from the enriched chewing gums was investigated during a chewing-like mechanism an‎d it was shown that if the gum was enriched with 0.8% of the complex, calcium release from it was significant. The results obtained from the present project indicate that these lipopeptide-calcium complexes can be effective in tooth remineralization an‎d, if added to chewing gum, can transform the chewing gum into a calcium delivery system to the tooth without significantly changing its properties. The use of these complexes can offer a non-invasive, practical an‎d promising solution for tooth regeneration using edible materials.

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پريناز صالحي

مهدي كديور , اميرحسين مهدوي