توصيفگر ها :
اكولوژي , بيماري ريشه گنايي , ايجاد اپيدمي , ژنوم ويروس , اپي تپ , خالص سازي , شبه قارچ , آنتي سنس , آنتي بادي منوكلونال , ايمن سازي , امتزاج الكتريكي , انجام فيوژن , انتخاب هيبريدوما , الكتروفورز , روش اليزا , آزمون وسترن بلات , ژل آگارز , روش ايمن , هيبريدوماهاي مثبت
چكيده انگليسي :
Abstract Rhizomaniais the most importantdisease sugarbeet leading to severedamage ofon sugar industry of our country every year This diseaseis causedby Beet NecroticYellow Vein Virus BNYVV which is transmitted by zoosporesof the soil bornefungus likepolymyxa betaeKeskin One of the most important and easiest methodsfordetecting infected plants is serological assay including the use of monoclonalantibodies mAb One of the currentmethodsto producemouse mAb is fusion of Bcells and Myeloma cells which are from the sameorigin In this study femaleBALB cmice were immunized with recombinantprotein CP21 which is the major coat proteinof the BNYVV Spleencell from immunizedmice about 108 were fusedwith mouseSP2 0 Ag 14myeloma cells 2xl07 at a ratio of 4 1 using 50 polyethyleneglycol PEGI500 The fusion mixture was suspended in HAT Hypoxanthine Aminopterin Thymidine selective medium After 2 weeks Supernatants growing ofhybridomas were screenedfor antibody production by ELISA Culture supernatantsshowing reactivity with CP21 were cloned by limited dilution and subcloned twice Further serological assaysand molecular methods showed that all hybrids producedantibodiesof IgG2a isotype with Kappa light chain The producedmAbs are also abletodetectinfectiousroots of sugarbeet Iiii
