2884

2748

كارشناسي ارشد

بيوتكنولوژي كشاورزي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1383

دوازده، 102، [II]ص.: مصور، جدول، شكل، نمودار

مسعود بهار، فردوس رستگار جزي

آقافخر ميرلوحي، فرشيد نور بخش

1384/4/26

احمد ارزاني، حسين شريعتمداري

1396/10/26

كتابنامه

كشاورزي

مهندسي كشاورزي

ID2748

به فارسي و انگليسي: قابل رويت در قسمت نسخه ديجيتالي

Abstract Analysis of the relationship between rhizobia that interact with the roots ofleguminose plants and induce the formation of nodules in which the fixation ofatmospheric nitrogen occures has been concerned since many years ago due to theirimportance for improvement of plant growth The specifity of the interaction betweenrhizobia and plants depends on many factors regulated for by the host cultivar andbacterial genome Therefore scientists in different countries are interested to understandthe details of relations between cultivars of one legume and populations of rhizobiaexist in divergent geographical area Until now various phenotypical and molecularmethods have been used to examine genetic relationship among rhizobia isolated fromdifferent plant host grown in diverse locations Because of importance of alfalfa as avaluable field crop study on the diversity of the strains of its simbioant Sinorhizobiummeliloti from different cultivars have been considered continuosly This study focusedon investigating the genetic structure of natural populations of S meliloti in western partof Iran in association to the local cultivars of alfalfa In this regard 35 soil samples werecollected from different area of Ilam Kermanshah Kurdestan and Hamedan provinces The soil samples divided in three pots and one of three native cultivars of alfalfa Baghdadi Hamedani and Rahnani was grown in each pot Following nodulating soilrhizobia on the hosts 10 strains of rhizobia were isolated from plants developed in eachpot and finally a collection of local rhizobia containing 1050 isolates was provided Allthe strains were subjected to melanin production test and the results suggested that thegeographical location and the cultivar have no significant affect on formation of thispigment in rhizobial isolates To study the genetic relationship of strains ofSinorhizobium meliloti isolated from different cultivars grown in different soils a partof nifH gene was amplified by PCR and RFLP analysis of this sequence was carried outexamine genetic variation of the rhizobia collected Also BOX AIR primer was usedfor typification these strains Data obtained were analysised by software NTsys 2 0 atUPGMA with Jacard coefficient The results indicated that geographical location affectthe genetic variability of S meliloti However the role of cultivar in this variation wasnot significant To determine whether rhizopine catabolism activity which play animportant role in nodulation competition can be found among Iranian isolates ofS meliloti a primer pair mocAF mocAnestR were employed to screen the strainscollected in this study Of the DNA extracts of 300 isolates examined four strains5A1 5A10 18C4 and 26C2 produced a 550 bp fragment which leads to recognize thesestrains as rhizopine catabolising rhizobia Comparision of the sequence of mocA regionin local rhizobia with the sequence of S meliloti L5 30 deposited in Blastn databaseshowed a high similarity 98 of Iranian isolates with L5 30 in mocA region Thisinformation confirms the conservation of mocA gene among strains of S melilotiisolated from different area in the world

مسعود بهار، فردوس رستگار جزي

آقافخر ميرلوحي، فرشيد نور بخش

احمد ارزاني، حسين شريعتمداري