توصيفگر ها :
قارچ خوراكي دكمه اي، تكمه اي , اسپان , كمپوست , پنچه دواني , بيماريهاي فيزيولوژيك , نشانگرها , اتصال آذاپتور , الكتروفورزژل اگاروز , تك انزيمي , كشت داوه , الادوخت , مالت،آگار , آرد، ذرت , ميكروسكوپ الكتروني , نگاره , ژنومي , روش موتامينتاشيكي، چن , كلريد منيزيم , بافر , ضريب كوفتنيك , آزمون مانتلر
چكيده انگليسي :
AbstractSince industrial production of button mushroom Agaricus bisporus in iran the greenmold epidemics by Trichoderma species caused setious damage Trichoderma spp colonizes compost and results in crop loss In this study sampling has preformed invarious stages from pili compost seed and soil and 630 samples were collected from 13industrial mushroom production units in Isfahan Char Mahal Bakhtiari Lorestan andHamedan provinces Trichoderma selective media and potato dextrose agar PDA wasused to isolate Trichoderma species Four hundred and twenty three isolates wereobtained and used for further study Microscopic features of conidiophores and shapeand size of conidia and colony characters and growth rate were observed on PDA SNAand CMD in different temperature 20 25 30 35 40C after 24 48 64 and 72 hours Outof 423 isolates three sections of Trichoderma Trichoderma Pachybasium Longibrachiatum were identified Among these isolates more than 350 related toTrichoderma sec 35 to Pachybasium and 20 related to Longibrachiatum section andT harzianum T virens T atrovirede T citrinoviride T ghanens andT longibrachiatum were identified from Agaricus bisporus T ghanense was reportedfor first time from Iran According to morphological and molecular studies isolate No 48 was different from other described species of Trichoderma and seemed to be a newspecie and Agaricus bisporus is Matrix Nova for T virens Combined molecular data sequence of internal transcribed spacer 1 and 2 and 5 8 S gene of rRNA PCR RFLPwith ITS1 ITS2 5 8S region and tef1 RAPD and AFLP morphology and colonycharacterization were used to study Trichoderma isolates According to AFLP andmorphology analysis it is concluded that application of these markers result sameclustering and also genetic diversity in T harzianum aggregate group proved by AFLP So we can say that AFLP is suitable marker for molecular genetic classification ofTrichoderma genus PCR RFLP in some cases have different results from othermolecular and morphological markers that it may be influenced by limitation ofenzyme number and sequence length Due to time consuming and low preciseness ofmorphological studies AFLP and PCR RFLP are more precise and powerful marker inTrichoderma classification
