3022

2937

كارشناسي ارشد

بيماري شناسي گياهي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1384

[ الف]، دوازده، 107، [II]ص.: مصور، جدول، شكل، عكس ﴿رنگي﴾، نمودار

علي آهون منش، كرامت اله ايزدپناه

مسعودبهار

رضا زارعي، امير مساح

1395/12/07

كتابنامه

كشاورزي

مهندسي كشاورزي

ID2937

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

AbstractPotato leaf roll virus occurs worldwide and causes crop losses whereverpotatoes are grown Surveys for potato leaf roll disease were conducted infields selected from areas with intensive potato production in Isfahan Province Tuber and leaf samples were taken in the growing season of 2003 and 2004 Disease incidence was assessedas a percentage of total number of infectedplants determined by DAS ELISA protocol and using specific polyclonal antibody to detect PLRV in collected leaf samples Maximum and minimumincidence of PLRV belonged to Hormoz Abad and Dasht e Hanna respectively with 38 71 and 9 09 The mean incidence of PLRV in IsfahanProvince was estimated to be 22 66 Uneven distribution ofPLRV in infected tubers was verified by using DAS ELISA and RT PCR techniques The highestconcentration of virus occurred at heel end and followed by the eye s in roseend of tubers Addition of sodium sulphite in extraction buffer andmodification of thermal condition of RNA precipitation step in ethanol resultedin more efficient recovery of total plant RNA which is prerequisite forsuccessful application of RT PCR Serological methods like DAS ELISA cocktail ELISA and molecular techniques including RT PCR IC RT PCR andPt RT PCR were optimized and compared with each other for accuratedetection of PLRV directly in dormant tubers In order to investigate thereliability of DAS ELISA and RT PCR tests on 20 dormant tubers each onetested was then scored for PLRV infection by growing the tuber and testing theleaves by ELISA On the basis of final results ELISA underestimated thepresence of virus just in one out of 20 tubers examined RT PCR technique wasapplied satisfactorily for the detection of PLRV in dormant tubers as well asscreen house grow on tuber tests leaf tests Cocktail ELISA enhanced thesensivity of ELISA to detect PLRV in tubers resulting in 1 5 2 times increasein the differences between absorbance values of infected tuber samples and healthy controls as compared to those obtained in DAS ELISA The resultsobtained from 30 dormant tubers tested by cocktail and DAS ELISAseparately was compared to those taken from performance of the similartechniques on greenhouse grown leaves of the same tubers Finally the cocktailELISA results confirmed but DAS ELISA again underestimated in one case ofdirect detection of PLRV in dormant tubers In conclusion the underestimationof DAS ELISA was not more than 4 in this study This level ofunderestimation is obviously less than that reported by others IC RT PCRsystem in comparison to common format of RT PCR needed total RNAextraction was considered as simpler and more sensitive protocol for thedetection of PLRV directly in potato tubers Application of Pt RT PCR as anaccurate and low cost diagnostic tool to detect PLRV in dormant potato tuberswas reported for the first time 1 lif l II i JC 1t

علي آهون منش، كرامت اله ايزدپناه

مسعودبهار

رضا زارعي، امير مساح