پديد آورنده :
مرتضوي، الياس
عنوان :
بررسي تحمل شوري در كالوس ارقام برنج ايراني و انتقال ژن كولين اكسيد از به برنج
گرايش تحصيلي :
﴿اصلاح نباتات﴾
محل تحصيل :
اصفهان:دانشگاه صنعتي اصفهان،دانشكده كشاورزي
صفحه شمار :
چهارده، 164، [II]ص.: مصور، جدول، عكس، نمودار
يادداشت :
ص. ع. به فارسي و انگليسي
استاد راهنما :
آقافخرميرلوحي، بهزاد قره ياضي
استاد مشاور :
احمدارزاني، نيراعظم خوش خلق سيما
توصيفگر ها :
واردات و صادرات برنج , پيچيدگي فيزيولوژيك , هوميوستازي يوني , اسموليت ها , گلايسين بتائين , ژن كولين اكسيداز , آرتروباكتر , بتائين آلدهيدوهيدروژناز , اي.كولاي , تجمع پرولين , آناليز سادرن
استاد داور :
مختار جلالي، هوشنگ عليزاده، بدرالدين ابراهيم سيدطباطبائي، علي محمدمير محمدي ميبدي
تاريخ ورود اطلاعات :
1395/08/25
كد ايرانداك :
ID142 دكتري
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
AbstractIn order to evaluate salt tolerance of four Iranian rice cultivars Zayandeh Roud TaromMolaie Neamat and Charam 2 and to select a cultivar for transformation using cholineoxidase gene which converts choline to glycine betaine a study was conducted inAgricultural Biotechnology Research Institute of Iran and College of Agriculture IsfahanUniversity of Technology In the first step in vitro salt tolerance of the cultivars was evaluatedusing a few experiments in which 6 levels of NaCl comprising as 0 20 40 60 80 and 100mM were added to MS Media Callus induction and growth and regeneration of plantlets fromcallus as well as Na K Ca Mg Cl ion content total sugars total soluble proteins andtotal proline content of dried callus and cell sap of callus were measured and analyzed Resultsrevealed that calli derived from cultivars Zayandeh Roud and Tarom Moline have the bestin vitro salt tolerance and use organic osmoregulatores less than other cultivars Moreover these cultivars have the best callus growth and regeneration making them good candidates forgenetic transformation Seed derived embryogenic calli of these cultivars were targeted inbiolistics method with plasmids pChlCOD and pCytCOD which contain choline Oxidase geneand nptII gene as selectable marker The first plasmid uses a transit peptide sequence to sendgene product to chloroplast resulting to accumulation of glycine betaine in chloroplast andother plasmid lacked this sequence resulting in sytosolic accumulation of glycine betaine Calli that had been selected on media containing 50 mgl 1 hygromycin developed into putativetransgenic seedlings All six plantlets transformed from pChlCOD and five plantletsbombarded with pCytCOD were PCR positive Southern analysis on four putative transgenicplantlets showed that at least a copy of choline Oxidase gene pChlCOD were integrated intogenome of the plantlets RT PCR analysis revealed that the gene was transcripted in at leastthree These putative transformed plant showed partial sterility but a few seeds achieved fromplants no 3 5 and 6 Some of these T1 seeds were germinated and grown PCR analysis forthese plantlets showed that the gene was inherited to the next generation More molecular andfunctional analyses are needed in T1 and in the next generations to estimate the copy numberof the gene transferred and to confirm transcription and translation of choline Oxidase and itseffect in enhancement of salt tolerance
استاد راهنما :
آقافخرميرلوحي، بهزاد قره ياضي
استاد مشاور :
احمدارزاني، نيراعظم خوش خلق سيما
استاد داور :
مختار جلالي، هوشنگ عليزاده، بدرالدين ابراهيم سيدطباطبائي، علي محمدمير محمدي ميبدي
