3231

3158

كارشناسي ارشد

﴿بيوتكنولوژي كشاورزي ﴾

اصفهان : دانشگاه صنعتي اصفهان ، دانشكده كشاورزي

1384

پانزده ، 152 ، [II] ، ص: مصور ، جدول ، نمودار ، شكل

ص . ع : به فارسي و انگليسي

سيروس قبادي ، كرامت الله ايزد پناه

مسعود بهار ، امير مساح

1395/08/25

مهندسي كشاورزي

ID3158

به فارسي و انگليسي : قابل رويت در نسخه ديجيتالي

HIAbstract Sugar is one type of nutrients with much of calorie which is needed for humanbeings Sugar beet is one of the important resources for producing sugar Growingof sugar beet as a strategic plant was be mentioned by who are responsible andobligate agriculture in Iran while pests and plant disease exist in the farthestreaches of the country Plant diseases are important factors which decrease yieldof sugar beet and Curly top disease is common disease for this plant Control ofthis disease with Using of efficient methods must be considered because it hasspreaded in much area in our century Gene silencing and RNA silencing in plantare useful methods With the purpose of making the inducing gene silencingconstructs 133 nucleotides were colonized from the end of rep gene of virus withtwo designed primers Rep gene F and Rep gene R in PCR reaction and intoPGEM T easy vector The inducing gene silencing constructs Z1 Z2 Z3 and Z4 were designed in plant Then silencing constructs were transferred toAgrobacterium tumefaciens GV3101 Two commercial varieties of sugar beet IC1and 9597 were used for gene transformation using Norouzi method Silencingconstructs were transferred to sugar beet plants which had 2 4 leaves with injector Results showed that the amount of symptoms of this disease were decreased bysilencing constructs Therefore using of inducing gene silencing constructs can bementioned as one of the best method for plant resistance to Curly top disease

سيروس قبادي ، كرامت الله ايزد پناه

مسعود بهار ، امير مساح