پديد آورنده :
فرهودي ، مجيد
عنوان :
رديابي و شناسايي ژنهاي مسوول توليد آنتي اكسيدانها در انار با استفاده از تكنيك DDRT- PCR
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
﴿ بيوتكنولوژي كشاورزي ﴾
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
سيزده ، 134 ، [II] ص: مصور ، جدول ، نمودار
يادداشت :
ص. ع. به فارسي و انگليسي
استاد راهنما :
بدرالدين ابراهيم سيد طباطبايي
استاد مشاور :
ضياء الدين طباطبايي
توصيفگر ها :
گياه شناسي انار , تكنيك DIFFERNTIAL DISPLAY , واكنش PCR , استخراج ، جداسازي RNA
استاد داور :
جواد كرامت ، سيروس قبادي
چكيده فارسي :
به فارسي و انگليسي : قابل رؤيت در نسخه ديجيتالي
چكيده انگليسي :
AbstractIran is one of the leading pomegranate producing countries in the world and has vastcultivar variation Pomegranate is an important source of bioactive components and hasbeen used in traditional medicine in many cultures It has been proved recently thatpomegranate juice and fermented pomegranate juice have high antioxidant activity All ofthese activities may relate to different phenol components which are present inpomegranate extract including punicalagin isomers derivatives of ellagic acid andanthocyanins Delphinidin Cyanidin 3 glucoside Pelargonidin and 3 5 di glucosidederivatives These components are famous for their free radical removing and oxidationinhibiting activities Extract derived from pomegranate peel in different concentrationshowed antioxidant and anti mutagenic characteristics and confirmed it s use as preserverand neutraceutical in food industry In comparison with peel pulp and seed in 28 kinds offruits pomegranate peel showed higher antioxidant activity Detection and recognition ofgenes of antioxidant biosynthesis pathway in pomegranate peel is considered in thisstudy Two methods have been performed to reach to this purpose 1 DDRT PCR technique or Differential Display RT PCR that is one of most efficient techniques in gene detecting 2 Designing primers for some of important genes of antioxidants biosynthesis pathway considering biosynthesis pathway and gene sequences recognized in other plants Production pathway of these products antioxidants have been studied in some plants that estimation of results has been used as a general pattern for selecting kind of genes forinvestigation and comparison In this project in addition to getting valuable ideas for nextstudies successful results in separating and isolation gene fragments were obtained
استاد راهنما :
بدرالدين ابراهيم سيد طباطبايي
استاد مشاور :
ضياء الدين طباطبايي
استاد داور :
جواد كرامت ، سيروس قبادي
