3314

3179

كارشناسي ارشد

﴿ اصلاح نباتات ﴾

اصفهان : دانشگاه صنعتي اصفهان ، دانشكده كشاورزي

1385

ده ، 108 ، [ II ] : مصور ، جدول ، نمودار ، عكس

ص . ع . به فارسي و انگليسي

فخر ميرلوحي ، بدرالدين ابراهيم سيد طباطبايي

بهرام شريف نبي

قدرت اله سعيدي ، غلامرضا بلالي

مهندسي كشاورزي

ID3179

به فارسي و انگليسي: قابل رؤيت در نسخه ديجيتالي

Abstract Symbiotic relationship between some members of Poaceae family has beendiscovered and a group of fungi called endophyte These symbionts belong to famillyClavicipitaceae which before were known as simple parasites of plant These fungigrow intercellulary in leaf sheets of host plants then enter to the seed through the ovaryand stay in aleuron layer Presence of these fungi in plant leads to resistance to variousbiotic and abiotic stresses Keeping these beneficial populations in host crops may leadto persistant productions during years and in different environments and will providescientists with new resources of germplasm for selecting plants for resistance to variousstresses In this study the endophyte fungi were isolated from leaf sheets of host plants F arundinacea F pratensis and L prenne Morphological characteristics of thesefungi were examined on PDA media To confirm Neotyphodium genus IS1 IS3 primerswere used AFLP molecular marker with 11 primer combination for plant genotypes and8 for fungi was applied Most of the isolates were morphologically similar to generaldescription of Neotyphodium The primer set IS1 IS3 amplifies a 444bp fragment withthis genus Based on morphological and molecular characters the genus Neotyphodiumon F arundinacea F pratensis and L prenne was identified The molecular resultsindicated that AFLP marker is able to discriminate between plant genotypes accordingto the species and geographic population Endophyte isolates were clustered into threeseparated groups Fungi species clustering was in consistent corresponding to plantspecies clustering Except for two isolates this was seen in the level of geographicpopulations Reciprocal comparing between two dendrograms resulted from AFLP datademonstrate high host specifity in the isolates used in our study Since AFLP can notreveal genetic differences intra populations strongly It is better to use SSR marker toconfirm the accuracy of the results

فخر ميرلوحي ، بدرالدين ابراهيم سيد طباطبايي

بهرام شريف نبي

قدرت اله سعيدي ، غلامرضا بلالي