پديد آورنده :
اميني، فاطمه
عنوان :
بررسي تنوع ژنتيكي در ژنوتيپ هاي مختلف گلرنگ با استفاده از صفات مورفولوژيك و نشانگر مولكولي RAPD
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
﴿ اصلاح نباتات ﴾
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
سيزده، 106، [II] ص.: مصور، جدول، نمودار
يادداشت :
ص. ع. به فارسي و انگليسي
استاد راهنما :
قدرت الله سعيدي، احمد ارزاني
استاد مشاور :
عبدالمجيد رضايي
توصيفگر ها :
نشانگرهاي ژنتيكي , واكنش PCR
استاد داور :
آقا فخر مير لوحي، بدرالدين ابراهيم سيد طباطبايي
تاريخ ورود اطلاعات :
1396/02/30
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتال
چكيده انگليسي :
Abstract To study the genetic variation of agronomic and morphological traits in 32 genotypes ofsafflower an experiment was conducted in a randomized complete block design with threereplications at Research Farm of Isfahan University of Technology located in Lavark Najaf Abad Also genetic diversity of 20 of those genotypes was assessed by RAPDmarker Agronomic and morphological traits of days to 50 germination days toflowering days to 50 flowering days to maturity plant height branches per plant capitula per plant seeds per capitulum 1000 seed weight reaction to powdery mildew seed yield and seed yield per plant were measured Analysis of variance showed thatgenotypes significantly differed for all studied traits Seed yield and seed yield per plantamong the genotypes varied from 1088 to 2168 kg ha and from 7 15 to 16 43 g plant respectively The highest and lowest genetic variation was observed for seeds percapitulum and days to maturity respectively Genetic and phenotypic correlationcoefficients showed that number of seed per capitula and number of capitula per plant hadthe high positive correlation with both seed yield and seed yield per plant Regressionanalysis also showed that number of capitula per plant explained 43 6 and along withseeds per capitula and plant height 60 of total variations for seed yield The results ofregression analysis for seed yield per plant revealed that seeds per capitula capitula perplant and 1000 seed weight explained 81 2 of its variation According to the results ofpath analysis capitula per plant had the most direct positive effect on seed yield and seedyield per plant however its effect was declined with indirect and negative effect of seedweight Principle component analysis revealed 6 factors contributing in 90 of totalvariations of the traits Factor analysis also recognized 3 factors which explained 81 81 of total variations These factors were named as yield components and plant height phonological traits and branch factor Cluster analysis and analysis of variance base on theagronomic and morphological traits grouped the genotypes in to 3 clusters The RAPDmarkers revealed 15 polymorphic primers out of 50 tested primers and percentage ofpolymorphism varied from 37 to 100 percents Similarity matrix revealed the highestsimilarity between Ac Sunset and Ac Sterling genotypes and the lowest similaritybetween E2428 and GE62923 genotypes C cluster analysis based on RAPD marker thegenotypes clustered in to 5 groups using 54 coefficient of similarity Comparing theresults of clustering based of morphological markers with those of molecular markersshowed that there were some differences Thees differences may be caused byenvironmental effects as well as amplification of non coding regions in molecularmarkers The existing of high variation for morphological traits and polymorphism atDNA level showed that some appropriate genotypes can be selected for safflower breedingprograms
استاد راهنما :
قدرت الله سعيدي، احمد ارزاني
استاد مشاور :
عبدالمجيد رضايي
استاد داور :
آقا فخر مير لوحي، بدرالدين ابراهيم سيد طباطبايي
