3678

3478

كارشناسي ارشد

بيوتكنولوژي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1386

شانزده، 122،[II] ص: مصور،جدول، نمودار

ص.ع.به فارسي و انگليسي

رضا زارعي، شهرام دخاني

محمد سراجي

10/6/86

بدرالدين ابراهيم سيد طباطبايي، نفيسه نيلي

مهندسي كشاورزي

ID3478

به فارسي و انگليسي: قابل رويت در نسخه ديجيتال

AbstractPomegranate is an important source of bioactive compounds that has been used forusual consumption as well as folk medicine for many centuries This fruit contains highamount of phenolic compounds with health benefits such as antioxidant anticancer antiatherosclerotic and neutraceutical properties Anthocyanins a subgroup offlavonoids of phenolic are known to be responsible for the pomegranate peel and fleshcolor In the present study total phenolic contents TPC and anthocyanin profiles ofred black and white peel pomegranates of 23 varieties obtained from Isfahan andYazd provinces were characterized The TPC of peels and seeds were extracted usingSoxhlet extractor with MeOH and determined according to the Folin Ciocalteuprocedure TAE Data were analyzed statistically using analysis of variance anddifferences among the means were determined for significance at P 0 01 using Leastsignificance difference LSD test and the system programmer SAS v 8 TPC of peeland seed ranged 37 to 176 1 and 0 84 to 2 5 mg g of dry material respectively and injuice 0 95 to 3 78 mg ml Highest TPC in juice peel and seed extracts were found incultivars Sefide e Ilam white peel Piyazi Ghasr e Shirin red and Shirin e PustSiyah e Yazd black cultivars respectively It was concluded that there were no clearrelationship between skin color and phenolic contents in cultivars that we analyzed Nevertheless results indicated that peel extract possessed high amount of phenolic compared to seed 64 times more Anthocyanins of the skin were also extracted andanalyzed by Thin layer chromatography TLC followed by High performance liquidchromatography HPLC equipped with DAD and mass spectrometry For TLC asolvent system comprising of butanol acid formic water hexan 40 15 40 5 wereselected amongst 21 other combinations for its resolving power for anthocyanins Usingthis system no anthocyanins were detected in white pomegranate although peels of redand black pomegranate were found to contain abundance of various cyanidin andpelargonidin derivatives In addition we identified a delphinidin in black pomegranatepeels To our knowledge this is the first report of this compound in pomegranate peel

رضا زارعي، شهرام دخاني

محمد سراجي

بدرالدين ابراهيم سيد طباطبايي، نفيسه نيلي