اندازه گيري برخي از تركيبات دارويي توسط ولتا متري عاري سازي جذبي در حد مقادير كم و مطالعه بر هم كنش كمپلكس مورين

چكيده انگليسي :

Abstract A new sensitive electrochemical procedure is presented for the determination of Rutinbased on the accumulation of its complex with Cu II onto the surface of hanging mercurydrop electrode for 80 s Then the preconcentrated Rutin complex was analyzed by cathodicstripping square wave voltammetry The effect of various parameters such as pH concentration of copper accumulation potential accumulation time and scan rate on thesensitivity were studied The optimum conditions for determination of Rutin include pH 6 0 6 0 ng mL 1 copper II concentration accumulation potential of 0 50 V and scan rate of0 40 V s 1 Under the optimum conditions and for an accumulation time of 80 s themeasured peak current at about 0 03 V is proportional to the concentration of Rutin overthe range of 2 0 82 0 nM The practical limit of detection is 1 5 nM The method wasapplied to the determination of Rutin in synthetic tea and pharmaceutical samples withsatisfactory results The diffusion coefficient complex formation constant and number ofelectron transfer of Rutin Cu II complex was also estimated A sequential method is proposed for the determination of tryptophan and histidine byadsorptive cathodic stripping voltammetry using standard addition and H point standardaddition method HPSAM The complexes of copper II with the amino acids wereaccumulated onto the surface of a hanging mercury drop electrode for 60 s Then thepreconcentrated complexes were reduced by square wave voltammetry and the peakcurrents were measured The effect of various parameters such as pH concentration ofcopper accumulation potential accumulation time and scan rate on the sensitivity werestudied by one at a time and artificial neural network Under the optimized conditions thepeak currents at about 0 05 to 0 30V is proportional to the concentration of tryptophanand histidine over the concentration ranges of 5 220 and 100 1200 nM respectively Optimization of the parameters by one at a time showed that at accumulation potential of0 10V versus Ag AgCl reference electrode the peak current is proportional only to theconcentration of tryptophan and histidine does not have any contribution to the current Theoptimization results by artificial neural network showed that at accumulation potential of 0 06V versus Ag AgCl the peak current is proportional to the both concentrations oftryptophan and histidine Therefore the method of H point standard addition has been usedfor resolving overlap voltammograms for determination of histidine in the present oftryptophane The method was successfully applied to the determination of tryptophan andhistidine in synthetic and real samples A reliable highly selective and sensitive procedure is presented for simultaneousdetermination of Captopril and Thioguanine based on cathodic adsorptive stripping ofCu I Captopril and Cu I Thioguanine complexes on a hanging mercury drop electrode Captopril and thioguanine were preconcentrated onto the surface of hanging mercury dropelectrode using Cu II as a suitable probe with accumulation time of 90 sec Then thepreconcentrated complexes were analyzed by cathodic stripping differential pulsevoltammetry The effect of various parameters such as pH concentration of copper accumulation potential accumulation time and scan rate on the sensitivity were studied The optimum conditions for simultaneous determination of captopril and thioguanineinclude pH 3 5 45 0 ng ml 1 copper II concentration accumulation potential of 0 10 Vand scan rate of 0 06 V s 1 Under the optimum conditions and for an accumulation time of90 s the measured peak currents at about 0 15 V and 0 4 V vs Ag AgCl areproportional to the concentrations of thioguanine and captopril over the ranges of 0 15 180and 0 5 100 0 nM respectively The limits of detections are 0 03 and 0 2 nM forthioguanine and captopril respectively The method was applied to the determination ofcaptopril and thioguanine in synthetic mixed samples pharmaceutical samples and humanserum with sati

حاجيان ،رضا

اندازه گيري برخي از تركيبات دارويي توسط ولتا متري عاري سازي جذبي در حد مقادير كم و مطالعه بر هم كنش كمپلكس مورين - بيسموت(III)باDNA

روتين , تريپتوفان , هيستيدين , كاپتوپريل , تيوگوانين , لوزارتان , تريامتون , منحني كاليبراسيون