3801

3593

كارشناسي ارشد

بيوتكنولوژي

اصفهان : دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1386

سيزده، 91، [II]ص.: مصور، جدول، نمودار

ص.ع. به فارسي و انگليسي

بدرالدين ابراهيم سيد طباطبايي، نعمت الله اعتمادي

1386/10/12

بهرام شريف نبي،خورشيد رزمجو

مهندسي كشاورزي

ID3593

به فارسي و انگليسي:قابل رؤيت در نسخه ديجيتال

AbstractTurfgrasses are the most common plants in landscapes Bermudagrass Cynodondactylon L Pers has a great importance because of it s resistance to drought salinityand traffics Iran is one of the centers of diversity and development of this plant Therefore subtle evaluation of these rich resources regarding morphological traits anddetermination of genetical diversity among them for doing breeding activities andcausing more qualified cultivars would be useful This study evaluated geneticaldiversity among 23 bermudagrass and four hybrid cultivars Tifdwarf Tifgreen Tifway Midlawn using morphological traits and ISSR molecular markers and then the best ofaccessions were selected for breeding activities Fourtheen quantitative morphologicaltraits and 20 qualitative morphological traits were respectively plant height rhizomediameter flag leaf length and width internode gap length and number of spikelets number of nodes below main inflorescence axis length of hair in flag leaf s collarregion length of upper glume and down glume length of lemma and palea width offlag leaf s ligule and fleshy rhizomes hairy node hairy stem in collar region softnessof leaf inflorescence colour spikelet state hairy florettail similarity of upper and downglume shape of upper and down glume hairy upper and down glume margine shape ofpalea and lemma hairy palea and lemma margine statement of lemma s ending flagcolour and days to flowering were measured After all 14 primers were used for ISSRexperiments Cluster analysis divided genotypes to three group basing on quantitativemorphological traits and to four groups basing on qualitative morphological traits andalso to four groups basin on ISSR molecular markers It was considered that in firstdendrogram which was based on quantitative traits four hybrid cultivars werebelonged to the same group with 4 75 Euclidean distance and these four hybrid cultivarsbelonged to the same group in second dendrogram with 4 75 Rogers distance as well Furthermore existing of two Iranian diploid accessions 17 and 88 in same group with5 Euclidean distance in the first dendrogram and with 9 Rogers distance in the seconddendrogram enforced the probability of being derived from common basement Incluster analysis of ISSR moleqular markers Midlawn hybrid cultivar which are coldhardy compared with Tif series was separated from hybrid cultivars of Tif series Accessions of each group located in this dedrogram had similarity ploidy levels Resultsof this analysis to main phenomenons of ISSR molecular markers showed that thesemarkers are dispersed on genome level Investigation of correlation between molecularand morphological coeffenetic matrixes showed that only between similarity matrixes oftwo grouping basing on quantitative and qualitative morphological traits a significantrelationship at 5 existed After calculating average genetical distances which wasresulted from investigating quantitative and qualitative morphological and moleculartraits respectively 27 19 24 53 13 18 39 genotypes and 39 18 13 53 24 19 27genotypes and 39 19 52 24 genotypes were selected Finally among these genotypes 39 19 24 genotypes common genotypes in each three groups were recommended forbreeding activities

بدرالدين ابراهيم سيد طباطبايي، نعمت الله اعتمادي

بهرام شريف نبي،خورشيد رزمجو