3809

3601

كارشناسي ارشد

علوم دامي

اصفهان : دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1386

يازده، 84، [II]ص.: مصور، جدول، نمودار

ص.ع. به فارسي و انگليسي

حميد رضا رحماني، محمد علي ادريس

قرباني، طباطبايي

12/10/86

خوروش، ميرلوحي

مهندسي كشاورزي

ID3601

به فارسي و انگليسي:قابل رؤيت در نسخه ديجيتال

AbstractMilk production and composition are affected by many different factors includinggenetic and environment In the physiology part growth and differentiation are resultof gestation and milking and causes to mammary gland secretion Peak of milkingperiod and its duration are in point of selection In dairy cows breeding with geneticselection that is enhancer of milking peak and persistency we can increase frequencyof favorite alleles that affect selected traits Candidates in somatotropic axis have animportant role in this way These candidates have polymorphisms in the part of theirgene that determine genotype of this polymorphism which can be used in the geneticselection One of these candidates is IGF I that is very important in growth differentiation and also in apoptosis and involution of mammary gland cells and it canalso act on milk production and compositions indirectly The objective of this studywas to determine IGF I polymorphism gene and its relation with Holstein dairy cow sperformance For the experiment blood was collected from 250 Holstein cows of fourdifferent herd managements in Isfahan province Genomic DNA was extracted fromwhole blood with salting out procedure Genotypes of IGF I were identified withPCR RFLP technique The polymerase chain reaction was used to amplify the 249 bpDNA fragments from genomic DNA PCR products were digested with SnaBIenzyme In this reaction with the presence of T nucleotide in the regulatory region ofthe gene 512 bp upstream of the initiation codon 249 bp DNA fragments convert to226 and 23 bp DNA fragments and it named A allele and with point mutation T to Cnucleotide this fragment remain without change Reaction digest productions weredistinguished on polyacrylamide gel and AA AB and BB genotypes were recognizedand then their frequencies determined B allele with 0 542 and AB genotype with0 630 had most frequencies in this study After that with GLM procedure of the SASprogram genotype and herd effects as fix effects and milking days open days and drydays as covariates we determined the relation between genotype and production rawmilk twice mature 305 days milk and composition raw fat and protein percentageof fat and protein and twice mature 305 days fat and protein milk reproduction gestation period days calving interval and calving interval to first insemination andbirth weight traits Through of these traits only percentage of fat and protein hadsignificant relation with genotype p 0 1 and p 0 05 respectively AB genotype had moreeffect than other ones Thus if the purpose of selection is increase of fat and proteinpercentage we can have deserve selection with this information

حميد رضا رحماني، محمد علي ادريس

قرباني، طباطبايي

خوروش، ميرلوحي