4065

3843

كارشناسي ارشد

بيماري شناسي گياهي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1386

دوازده، 87، [II] ص.: مصور، جدول، نمودار

ص. ع. به فارسي و انگليسي

مسعود بهار

محمدرضا لك

05/05/87

امير مساح، احمد ارزاني

1396/03/06

كتابنامه

كشاورزي

مهندسي كشاورزي

ID3843

به فارسي و انگليسي: قابل رؤيت در نسخه ديجيتال

AbstractCommon bacterial blight has become more prevalent in many bean growing areas ofIran To gain insight into the nature of these outbreaks it is essential to understand thepathogenic variation and genetic diversity among strains of the disease causal agent Xanthomonas axonopodis pv phaseoli Xap dispread across the country For thisreason strains of Xap isolated from beans growing sites in Lorestan Markazi Isfahan Charmahal Bakhteyari and Zanjan provinces were initially characterized according totheir colony morphology biochemical and serological properties The isolates thosewere confirmed as Xap were further characterized by polymerase chain reaction PCR using species specific primer pair X4c X4e which leads to the amplification of a 730bp DNA fragment The generated fragment from different strains was digested withrestriction endonuclease Rsa I Taq I Hae III and Sau 96I In this PCR RFLP assay Identical DNA patterns produced by strains originating from different locationsconfirmed their homogeneity With exception of Xapf isolate the specific primer pairXf1 Xf2 did not amplify any fragment from Xap isolates A PCR based fingerprintingmethod with markers BOX PCR and ERIC PCR rep PCR method primers were usedfor genotypic characterization of Xap isolates and reference strains Xcpf Based upondata from rep PCR analysis the polymorphism rate among Xap isolates wasdetermined 15 79 and 19 for BOX PCR and ERIC markers respectively Geneticsimilarity between Xapf and Xap isolates was in a range 38 through 46 The rep PCR fingerprinting readily distinguished Xapf isolate from those of Xap isolates however despite a limited genomic differences among isolates the overall level ofpolymorphism within Xap isolates collected from different locations in Iran was low confirming their close relationship In order to investigate pathogenicity variation andtobacco hypersensitivity reaction among the isolates suspension of Xap and Xapfstrains were inoculated on pinto bean cv CFO6 and tobacco Nicotiana tobacum cv samsun Based on evaluating of pathogenicity severity and hypersensitivity reaction the isolates grouped as virulent and moderately virulent However the data did notreveal a relationship between genetic diversity and virulence differences of isolates Since Pathogenicity of strains can be an important parameter in virulence of commonbacterial blight agents the pathogenic variation among isolates which has beenconfirmed in this study should not be ignored As a consequence it is highlyrecommended to investigate the pathogenicity of these isolates on different beancultivars in Iran

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محمدرضا لك

امير مساح، احمد ارزاني