4071

3849

كارشناسي ارشد

بيماري شناسي گياهي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1386

سيزده، 93، [II] ص.: مصور، جدول، نمودار

ص. ع. به فارسي و انگليسي

بهرام شريف نبي، اكبر خداپرست

اكبر نكويي

5/5/87

علي آهون منش، غلامرضا بلالي

1396/03/03

كتابنامه

كشاورزي

مهندسي كشاورزي

ID3849

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Abstract Alfalfa and clover are two important forage crops of the family Fabaceae More overthey have useful effect on soil structure and have a fundamental role in feedingdomesticated animals As a consequence they influence on meat and milk production which influences on human nutrition The powdery mildow of alfalfa and clover is oneof the diseases in some regions of Iran In 1385 twenty isolates of powdery mildewswere collected from Isfahan province Furthermore one isolate of clover and one ofalfalfa powdery mildow from Zanjan were used Sixteen isolates were recognized asOidium spp as they have no mature ascocarp or even without it Two isolatesrecognized as Erysiphe pisi and three others recognized as E trifolii var trifolii Isolateof Baghbahadoran s clover 1 was identified as E trifolii var desmanthi which isreported for first time from Iran Five weeds were collected from inside of alfalfa andclover fields to test the ability of their infectivity on alfalfa and clover crossinoculation was done in greenhouse Only isolates of clover and alfalfa could infectclover and alfalfa and no infection from weed sites to clover and alfalfa was observed Considering these results powdery mildews of other collected hosts do not have any rolein infection of alfalfa and clover plants Six methods of DNA extraction have beentested and crushing of mycelia between two glass slides gave sufficient amount ofDNA ITS1 5 8s ITS2 regions of rDNA was amplified using primer pairs of ITS5 andP3 and in nested PCR assay ITS1 and P3 primer pairs was used A 522 bp fragment wasamplified in E pisi and 558 bp fragment was amplified in E trifilii PCR RFLP profileswere obtained using TaqI AluI MspI HinfI restriction enzymes The profiles from AluIconfirmed that different isolates are identical and the cluster analysis based on threeother enzymes showed variability among different isolates There was 75 percentsimilarity among different isolates PCR RFLP results was concordant withgeographical distribution of isolates and could separated the two species E pisi andE trifolii

بهرام شريف نبي، اكبر خداپرست

اكبر نكويي

علي آهون منش، غلامرضا بلالي