4073

3851

كارشناسي ارشد

بيوتكنولوژي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1386

دوازده، 97، [II] ص.: مصور، جدول، نمودار

ص. ع. به فارسي و انگليسي

سيروس قبادي، بدر الدين ابراهيم سيد طباطبايي

احمد يامچي

05/05/87

امير مساح، مصطفي مبلي

1396/03/03

كتابنامه

كشاورزي

مهندسي كشاورزي

ID3851

به فارسي و انگليسي: قابل رويت در نسخه ديجيتال

Abstract The commercial production of plant is growing worldwide Its monetary value has significantly increased over the last two decades and there is a great potential for continued further growth in both domestic and international markets Regenerated and transformed petunia plants Petunia hybrida cv Supercascaide Petunia hybrida cv Opera Petunia Nictaginiflora for Iranian and forein cultivar have been produced by means of shoot apex and leaf disc Agrobacterium mediated transformation method Seeds of different cultivars were grown in MS medium for regeneration via shoot apex Shoot apex were isolated from seven days seedlings and in order to select the best shooting media the statistical analysis explants in a CRD with 12 shooting treatments MS B5 vitamins with different concentration of NAA 0 0 2 0 5 and BAP 0 0 1 0 5 1 The statistical analysis indicated that the best for producing multiple shoot was medium with 1mg l BAP Optimizing regeneration via leaf disc explants of different cultivars were put in three modified MS media and Supercascaide leaf discs were put in 10 different medium that were different in concentration and kind of growth regulators The results showed that the best medium for direct regeneration was MS3 medium Of course mean comparisons between media and Petunia hybrida Opera Supercascaide and Petunia Nictaginiflora indicated that the best for producing direct shoot were Nictaginiflora species and Supercascaide cultivar in MS3 and MS12 respectively The best for producing indirect shoot was Opera in MS3 medium The leaf disc and shoot apex were co cultivated with Agrobacterium strain LBA4404 harboring a plasmid pBI121 was used as the vector system for transformation pBI121 contained glucuronidase gus gene as a reporter gene and pyroline 5 carboxilate synthase p5cs driven by the cauliflower mosaic virus CaMV35S promoter and neomycin phosphotransferase nptII gene used as a selectable marker The leaf disc and shoot apexes were transferred to selective medium containing Kanamycin 200 mg l and then transferred to proliferation medium modified MS with 1 mg l BAP The cultures were kept at 25 2 C and 16 8 hour photoperiod under cool white fluorescent light at 40 molm 2s 1 Shoot regeneration occurred within 3 weeks To confirm transformation regenerated plants were subjected to the polymerase chain reaction PCR GUS and proline assay confirmed the perscence of the gus and p5cs gene in the genome of all transformants in stress and nonstress conditions pdfMachine A pdf writer that produces quality PDF files with ease Produce quality PDF files in seconds and preserve the integrity of your original documents Compatible across nearly all Windows platforms if you can print from a windows application you can use pdfMachine Get yours now

سيروس قبادي، بدر الدين ابراهيم سيد طباطبايي

احمد يامچي

امير مساح، مصطفي مبلي