تعيين تنوع ژنتيكي ژنو تيپ هاي به ﴿Cydonia oblonga﴾ با استفاده از نشانگرهاي ريز ماهواره اي

چكيده انگليسي :

Genetic Diversity of Quince Cydonia oblonga Using Microsatellite Markers Moein Dehbashi m dehbashi ag iut ac ir modehbashi @ @ 110 gmail com March 14 2009 Department Agricultural College of Agricultural Biotechnology Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc language Farsi B E Sayed Tabatabaei Assoc Prof sayedt@cc iut ac ir M Bahar Assoc Prof mbahar@cc iut ac ir Abstract Quince tree Cydonia oblonga Mill as an individual species of this genus is one of the several kinds of pome fruits Although it s genuine origin has been unknown but it can be suggested that Iran Middle Asia Anatolia and Greece are as their probable origin rather than others This plant belongs to subfamily of Maloideae and family of Rosaceae For the first time in this research the genetic relationships of quince genotypes in Iran where is one of the possible origin has been studied In year 2007 Turkey has been known as the greatest quince producer in the world In year 2005 the quince cultivated plane in 26 provinces of the country is 5804 57 hectares and its quantity production has been estimated to be 39312 98 tons Isfahan has been shown as the greatest quince producer in Iran Following Isfahan Fars Kerman and Razavi Khorasan provinces have been ranked as the greatest quince producers of this product Having various ecological conditions Iran from the remote past had been suitable slaughter house for this plant For the present time there are not found any precise information about the genetic relationships of the quince genotypes in Iran and foreign varieties in the world In this study 20 genotypes were belonging to Isfahan and Razavi Khorasan provinces and three foreign root stocks including quince A B and C was employed Two methods on microsatellites including SSR Simple sequence repeat and ISSR Inter simple sequence repeat was conducted in order to determine their genetic relationships Because lack of any the special primers of this plant therefore 28 pairs of apple SSR primers have been used and amonge which the eight pairs of SSR have been shown with cross genus amplification and 10 loci middling produced 2 8 alleles The SSR markers dendrogram was obtained through using coefficient of Nei 1983 and method of UPGMA by Power marker software showed that the used genotypes in this research can be divided into Isfahan province s genotypes and Razavi Khorasan s ones and the three root stocks used were set in the group of Razavi Khorasan province s genotypes and those have shown the genetic affinity with respect to these genotypes The quince A with Neishaboor Deezbad with zero genetic distance was settled in one group Consequently the quince B having a little difference has shown a close relationship to quince A and it in company with Torbate jam genotype was set in one group Quince C was alone set independently in the group of Razavi Khorasan province s genotypes It seems as though that in the group of Razavi Khorasan province s genotypes two genotypes of Neishaboor borj and Neishaboor kharv are probable origins of two groups of Razavi Khorasan genotypes In the group of Isfahan province s genotypes the Semirom vanak 2 genotype and Falavarjan s ones are possibly considered as origin of genotypes in this group According to SSR results Isfahan province s genotypes have shown the most various genetic backgrounds in relation to Razavi Khorasan province s genotypes Out of 24 ISSR it was observed that 174 bands of total 253 bands produced 68 6 percent polymorphism The average polymorphism bands for every primers 7 25 bands were obtained Dendrogram obtained from ISSR primers by using Jaccard s similarity coefficient and UPGMA method designed by software NTSYS V Pc 2 02e and genotypes and root stocks which were used have been divided into two groups and one independent genotype Chenaran and therefore like SSR dendrogram no relation between geographical origin of genotypes in this division has been observed In comparison between SSR and ISSR

دهباشي، معين

تعيين تنوع ژنتيكي ژنو تيپ هاي به ﴿Cydonia oblonga﴾ با استفاده از نشانگرهاي ريز ماهواره اي

SSR , ISSR