4637

4365

كارشناسي ارشد

حشره شناسي كشاورزي

اصفهان،دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1387

چهارده،130،[II]ص.: مصور،جدول،نمودار

ص.ع. به فارسي و انگليسي

بيژن حاتمي، رحيم عبادي

88/7/4

بهرام شريف نبي،نعمت اله اعتمادي

1396/09/13

كتابنامه

مهندسي كشاورزي

ID4365

به فارسي و انگليسي: قابل رويت در نسخه ديجيتال

The Singular and Combined Effects of Entomopathogenic Fungi Beauveria bassiana Bals Lecanicillium muscarium Petch and Insecticide Imidacloprid on Different Nymphal Stages of Trialeurodes vaporariorum in the Laboratory Conditions Naser Malekan 11 3 2009 N malekan@ag iut ac ir Department of Agriculture Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiProf Bijan Hatami bhatami@cc iut ac irProf Rahim Ebadi ebadir@cc iut ac irAbstract Trialeurodes vaporariorum which belongs to the order Hemiptera and the family Aleyrodidae canlead to severe economic losses It s life cycle contians egg four larval and adult stages Fungi seem tobe the only entomopathogenes that may be used to control whiteflies because thay are able to penetratetheir hosts while other pathogens need some routes to enter their host Most fungal pathogens ofwhiteflies belong to the genera Aschersonia Beauveria Lecanicillium and Paecilomyces The aim ofthis project was to investigate the singular and combined effect of the entomopathogenic fungiBeauveria bassiana and Lecanicillium muscarium and imidacloprid insecticide for control ofgreenhouse whitefly Before commencing the transfer of greenhouse whitefly each tomato leaflet wascovered with a clip cage Approximately 40 adults randomly collected from a single population weretransferred into each cage They were then allowed to lay eggs on the leaflet for 72h at 24 1 C 70 10R H under 16 8 L D phoroperiod 11 days prior starting of experiment This time interval resulted infirst and second instar nymphs to appear and 20 days after all nymphs in third and fourth instarsappeared at the time of fungal inoculation The fungi were cultured in Petri dishes containing PDA inthe incubator In fungal bioassay conidial suspension was sprayed on detached leaves conidiasuspensions containing 103 106 conidia ml 1 was applied to the underside of each leaflet using a Potterspray tower with fine droplet spray nozzle in a randomized complete block design A control leafletwas treated with 2 ml of sterile distilled water containing 0 01 Tween 80 The dishes were incubatedin an incubator at 24 1 C 70 10 R H set under 16h light and 8h dark Insects that were either coveredby dense hyphal growth or whose colour changed from transparent greenish to opaque white or lightyellow were considered dead similarly The bioassay of imidacloprid was performed the same way asbioassay of entomopathogenic fungi The mean viability of the spores of the straines applied to all thedevelopmental stages in the parental generation was 95 5 The results showed that imidacloprid is notcompatible with L muscarium and it caused complete inhibition in fungal vegetative growth in 2 MC Mean Concentration but average daily growth increase of B bassiana was not significantly differentin comparison with the control Likewise germination of conidia at 2 MC treatment was not affectedwith imidacloprid while in L muscarium the rate of germinated conidia was reduced significantlycompared with the control Regarding lack of negative influence of imidacloprid on B bassiana acombination of these two can be recommended in integrated pest management programs L muscariumand B bassiana grew on a solid culture medium and maintained in the incubator with condition

بيژن حاتمي، رحيم عبادي

بهرام شريف نبي،نعمت اله اعتمادي