4999

4703

كارشناسي ارشد

علوم دامي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1388

سيزده،77ص: مصور،جدول

ص.ع. به: فارسي و انگليسي

حميدرضا رحماني، محمد علي ادريس

بدرالدين ابراهيم سيد طباطبايي

11/11/88

محمد خوروش، مجيد طالبي

مهندسي كشاورزي

ID4703

به فارسي و انگليسي: قابل رويت در نسخه ديجيتال

Estrogen Receptor Alpha Gene Polymorphism and it Relation with Reproductive Traits in Iranian Dairy Holstein Azadeh Zahmatkesh a zahmatkesh@ag iut ac ir November 1 2009 Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Dr H M Rahmani hrahmani@cc iut ac ir Dr M A Edris edriss@cc iut ac irAbstractTo provide human needs it is very important to produce more milk and meat from less number of animals Soimprovement in reproduction will lead to produce more milk in every one day of animal s longevity To reachthis purpose genetics and animal breeding are very essential There are a lot of genes affecting quantitativetraits so direct selection of these traits would be very difficult and complicated Finding a genetic marker whichphysiologically affects the quantitative traits expression or is in linkage with major genes with great influenceson reproductive traits may improve them by increasing selection accuracy and decreasing generation interval Estrogen is one of the major hormones that have a large effect on growth differentiation and function of manytissues such as reproductive tissues uterine ovary oviduct mammary glands testis and prostate So becauseof the estrogen s main role in regulation of reproduction it s alpha subtype receptor gene ER have been usedas a genetic marker for reproductive traits ER protein is distributed in many tissues of male and femalereproductive organs and has a key role in regulation of reproduction physiology In this study ER genepolymorphism in 5 region in consensus promoter for exon C A G transition and it s relation with reproductiveand productive traits and birth weight was investigated Genomic DNA was isolated from 200 dairy Holstein infour farms in Isfahan province by using Miller salting out DNA extraction procedure Then they weregenotyped by PCR RFLP technique as the DNA fragments containing the mutation were amplified usingspecial primers and then digested with BglI endonuclease enzyme Fragments with 245 bp represented allele Aand those with 168 bp and 77 bp were equivalent to allele G Allelic frequencies were 0 0742 and 0 9257respectively and the distribution of AA AG and GG genotypes was 0 010 0 129 and 0 861 respectively According to the calculations the studying population was in Hardy Weinberg equilibrium It means that this

حميدرضا رحماني، محمد علي ادريس

بدرالدين ابراهيم سيد طباطبايي

محمد خوروش، مجيد طالبي