5118

4808

كارشناسي ارشد

علوم دامي

اصفهان: دانشگاه صنعتي اصفهان،دانشكده كشاورزي

1388

هشت،59ص.:مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

نفيسه نيلي،عبدالحسين سميع

حميدرضا رحماني،ايرج كريمي

89/1/28

مهدي محمد علي پور،مهدي كديور

مهندسي كشاورزي

ID4808

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Effect of Diet Form and Energy on Ascites Incidence and Biochemicaland Histopathological Changes in Broilers Pulmonary Artery Mina Bagheri Varzaneh Mina b641028@yahoo com Date of submission December 15 2009 Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi N Nili Ph D Assistant Professor Supervisor nilin@cc iut ac ir A Samie Ph D Assistant Professor Supervisor asamie48@cc iut ac irAbstractHigh energy and pelleted diets increase growth rate and improve feed conversion ratio FCR in broilers However these diets can increase the incidence of metabolic disorderssuch as ascites The aims of this study were to investigate effects of pelleting or increasingthe level of energy in the diet on ascites incidence and biochemical and histopathologicalchanges in broilers pulmonary artery 608 male broilers ROSS 308 were used in acompletely randomized design Treatments n 4 included high 3200 kcal kg and low 2868 kcal kg from 7 21 day and 2955 kcal kg from 21 42 day levels of dietary energyeach combined with two forms of mash or pellet Chickens were sacrificed at 3 4 5 and 6weeks Histopatological analysis H E staining was performed on pulmonary artery Collagen and elastin were measured in pulmonary artery by cyanogen bromide treatment Gelatin zymography performed for detection of matrix metalloproteinases MMPs Datawere analyzed by GLM procedure of SAS A P value less than 0 05 was considered statisticallysignificant At 42 days there was no difference in arterial wall thickness medial thickness external diameter and lumen diameter between high energy pelleted diet HP low energypelleted diet LP and high energy mashed diet HM and low energy mashed diet control Right ventricular weight total ventricular weight was the lowest in control ascompared other treatments HP 0 16 0 007 LP 0 15 0 006 HM 0 16 0 007 vs control 0 12 0 006 Collagen content was higher in all diets than control HP 493 78 10 02 LP 483 02 9 14 HM 475 54 9 55 vs control 439 4 9 14 g cm Thesedata showed that high energy and pelleted diets increase collagen synthesis howeverelastin content did not change FCR improved in pelleted diets compared to mashed diets The activation of MMP 9 increased on 5 and 6 weeks at d 42 high energy pelleted dietresulted in biochemical changes at cellular molecular level Although treatments did notresult in incidence of clinical ascites pelleting the high energy diets can induce significantbiochemical changes that may eventually lead to ascites Key words energy density pelleted diet elastin collagen MMP 9 ascites

نفيسه نيلي،عبدالحسين سميع

حميدرضا رحماني،ايرج كريمي

مهدي محمد علي پور،مهدي كديور