5176

305 دكتري

دكتري

علوم دامي

اصفهان: دانشگاه صنعتي اصفهان،دانشكده كشاورزي

1388

دوازده، 100 ص.: مصور ﴿ رنگي ﴾، جدول، نمودار.

ص. ع. به فارسي و انگليسي

حميد رضا رحماني، نفيسه نيلي

عبدالحسين سميع، صبيحه سليمانيان زاد

22/2/1389

محمد علي ادريس، احمد زارع شحنه، علي اصغر ساكي

مهندسي كشاورزي

ID305 دكتري

به فارسي و انگليسي: قابل رؤيت در نسخه ديجيتال

Anti Bacterial Activities of Chicken Egg Yolk Antibody IgY on Escherichia coli O78 K80 and Passive Immunity Effect of IgY against Escherichia coli O78 K80 in Broilers Amir Hossein Mahdavi mahdavi@ag iut ac ir ahmahdavi2000@yahoo com 3 2 2010 Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 Iran Supervisors Dr H R Rahmani hrahmani@cc iut ac ir Dr N Nili nilin@cc iut ac ir Advisors Dr A H Samie Dr S Soleimanian Zad Department of Graduate Program Coordinator Dr F Nourbakhsh Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 Iran Department of Food Science and Technology Isfahan University of Technology Isfahan 84156 83111 Iran Abstract The in vitro and in vivo growth inhibitory effects of egg yolk antibody IgY powder was investigated on Escherichia coli O78 K80 as the major cause of associated septicemic disease in broilers E coli O78 K80 was incubated with IgY 50 100 and 150 mg ml specific sIgY and non specific nsIgY IgY powder for 6 h incubation period In vivo experiment was conducted with 392 chickens in a completely randomized design with 7 treatments six E coli O78 K80 oral challenged groups and one unchallenged group as negative control 4 replicates and 14 chickens per replicate The challenged groups received diets supplemented with 0 as positive control 0 1 0 2 and 0 4 sIgY from eggs of immunized hens and 0 2 and 0 4 nsIgY from eggs laid by the nonimmunized hens sIgY at the concentration of 150 mg ml decreased bacterial proliferation by 1 18 log cfu ml compared with the control group at 6 h of incubation period P 0 01 After three weeks of feeding the levels of 0 2 and 0 4 sIgY and 0 4 nsIgY had the most suppressive effects P 0 01 on the ileal E coli enumeration and intestinal secretory IgA concentration Dietary supplementation with at least 0 2 sIgY caused respected increase P 0 01 in the villus height to crypt depth ratio and depression in the heterophils to lymphocytes ratio P 0 05 the jejunal goblet cells and lamina propria lymphatic follicles numbers and feed conversion ratio P 0 01 Key words broiler chick egg yolk antibody IgY powder E coli O78 K80 oral passive immunity mucosal morphology Introduction During the recent years the extended research works have developed the approaches to prevent and control bacterial infections in the poultry industry These approached methods are include of improved hygienic methods vaccine applications use of competitive exclusion

حميد رضا رحماني، نفيسه نيلي

عبدالحسين سميع، صبيحه سليمانيان زاد

محمد علي ادريس، احمد زارع شحنه، علي اصغر ساكي