5257

4927

كارشناسي ارشد

شيلات

اصفهان: دانشگاه صنعتي اصفهان،دانشكده منابع طبيعي

1388

چهارده،103ص.:مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

نصرا..محبوبي صوفياني

امين نعمت الهي

18/3/89

عيسي ابراهيمي،يزدان كيواني

مهندسي منابع طبيعي

ID4927

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

116 The effect of probiotic prebiotic and their combination on growthperformance some intestinal morphology and microbial floor in therainbow trout Oncorhynchus mykiss Ebrahim motaghi Ebrahimmotaghi@yahoo Com Date of Submission 2010 04 04 Department of Natural Resources Isfahan University of Technology Isfahan 84156 83111 Iran Degree M S c Language Farsi Supervisor Dr Nasrollah Mahbobi Soofiani soofiani@cc iut ac ir Abstract Probiotics are live micro organisms which by improving intestinal microbial balance with health benefit thehost whereas prebiotics are non digestible oligosaccharides which by stimulating growth and microbial activityin intestin effects the well being of the host The present study was carried out to investigate an intestinalmicrobial balance growth performance and immune responses of fish trough addition of Gallipro probiotic andFermacto prebiotic to diet of rainbow trout Oncorhynchus mykiss In a completely randomized design with 3 3factorial arrangements 9 experimental diets counting three dietary levels of Gallipro 0 0 2 and 0 4 g kg diet three levels of Fermacto 0 1 and 2 g kg diet were used Each treatment was replicated 3 times Four hundredand eighty six 486 rainbow trouts with mean live weight 19 06 1 83 g were randomly selected and allocated to27 tanks 18 fish per tank The experiment was carried out for 10 weeks Results showed that 0 4 g kg diet ofGallipro improved the specific growth rate feed conversion ratio and weight gain at the end of experiment butFermacto showed the opposite effects on the similar criteria The lowest triglyceride cholesterol HDL and LDLof fish blood serum were observed in the diet that contained 0 4 g kg Gallipro but application of Fermactosupplement showed a significantly increasing effect on the similar items The highest increase in blood serumfactors were observed in diet that contains 2 g kg Fermacto Gallipro and Fermacto combination in dietsimproved fish blood serum factors significantly p 0 05 These supplements showed no significant effect on fishblood serum proteins Gallipro and Fermacto caused significant differences in blood serum lysozyme activity The diet containing 0 2 g kg Gallipro and 1 g kg Fermacto increased and 2 g kg Fermacto alone decreased bloodserum lyzosyme activity significantly The presence of Bacillus subtilis in diet resulted in a higher bacterialpopulation and improving fish intestinal microbial balance The highest bacterial population in fish intestine wereobserved in the diet that contained 0 4 g kg Gallipro and 1 g kg Fermacto Length of villous of fish intestinalepithelium was also affected by Gallipro and Fermacto The highest length of villous was observed in the dietthat contained 0 2 g kg Gallipro However the lowest length was observed in fish receiving the dietsupplemanted with 2 g kg Fermacto In addition Gallipro and Fermacto supplementation improved significantlythe length of villous This study showed that supplementation of 0 4 g kg Gallipro in the diet could improveperformance of juvenile rainbow trout whereas application of Fermacto at the applied levels showed no effectson growth and health of experimental fish Key words Probiotic Perbiotic Gallipro Fermacto Rainbow trout Microbial andlyzosim activity

نصرا..محبوبي صوفياني

امين نعمت الهي

عيسي ابراهيمي،يزدان كيواني