5272

4942

كارشناسي ارشد

بيوتكنولوژي كشاورزي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1388

دوازده،86ص.:مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

نفيسه نيلي،بدرالدين ابراهيم سيد طباطبائي

احمد موحديان عطار

23/3/89

حميدرضا رحماني،شقايق حق جوي جوانمرد

1396/10/02

كتابنامه

مهندسي كشاورزي

ID4942

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

The Effect of Anthocyanins of Pomegranate Skin on Atherosclerosis Plaque Formation in Rabbit Arteries and Proteomic Analysis Moloud Ahmadi Moloud ahmadi@ag iut ac ir Department of biotechnology college of agriculture Isfahan University of Technology Isfahan Iran Degree MSc Language Farsi Nafise nili nilin@cc iut ac ir Badraldin Ebrahim Sayed Tabatabaei sayedt@cc iut ac ir Abstract Pomegranate is an important source of bioactive compounds that has been used for usual consumption as well as folk medicine for many centuries This fruit contains high amount of phenolic compound with health benefits such as antioxidant anticancer and neutraceutical properties The antiatherogenic properties of pomegranate has been also attributed to its capacity to decrease oxidative stress the hallmark of early atherogenesis Anthocyanins a subgroup of flavnoids of phenolic are known to be responsible for the pomegranate peel and flesh color It has been shown that antioxidants increase number of EPCs and contribute to the attenuation of atherosclerosis plaque size The object of this project was the effect of pomegranate peel anthocyanins on atherosclerosis plaque formation and plaque proteomics analysis The anthocyanins of pomegranate peel were extracted using aciditic ethanol Total polyphenolic compounds were determined g l according to the Folin Ciocalteu procedure Antioxidant activity of extract was determined using DDPH system and this extract shows antioxidant activity in microgram Rabbits were assigned in groups by standard diet high cholesterol diet HC group n or high cholesterol diet with oral administration of an anthocyanin rich extract from pomegranate skin HC Pom group n for weeks Atherosclerotic plaque size was significantly reduced in anthocyanin fed rabbits in comparison to the control group and in threated group calcification was not observed Alteration of MMPs was analyzed by gelatine zymography The result of zymography shows increasing amount of MMP and MMP in cholesterol group in comparision to treated group and negative control EPC CD VEGF R circulating levels were evaluated in peripheral blood by Flowcytometry CD KDR cells were significantly higher in treated group than cholesterol group vs p By the end of this research we studied the difference of aorta proteins between tree groups with two dimentional electrophoresis and important difference between negative group and two other groups was observed In this study for the first time beneficial consequence of pomegranate skin anthocyanins in prevention of this disease was reported Dramatic alterations in the protein expression profile of aorta tissue was detected after the development of AS disease in rabbits Investigationof the relationship between the differentially expressed proteins andthe initiation and development of the AS would be crucial for theunderstanding of the mechanism s of the disease and possible discovery of novel diagnosis markers and therapeutic approaches Key words Pomegranat Antioxidant Anthocyanin Atherosclerosis Proteomics

نفيسه نيلي،بدرالدين ابراهيم سيد طباطبائي

احمد موحديان عطار

حميدرضا رحماني،شقايق حق جوي جوانمرد