5275

4945

كارشناسي ارشد

علوم باغباني

اصفهان: دانشگاه صنعتي اصفهان،دانشكده كشاورزي

1388

نه،68ص.:مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

نعمت ا... اعتمادي، سيروس قبادي

بدرالدين ابراهيم سيد طباطبائي

24/3/89

بهرام باني نسب، مجيد طالبي

1396/10/02

كتابنامه

مهندسي كشاورزي

ID4945

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

The Effects of Different Explants and Plant Growth Regulators on Production of Persian Buttercup Ranunculus asiaticus L Plantlets In vitro Zohreh Poorshafie www z poorshafie@ag iut ac ir March 22 2010 Department of Horticulture Science Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi 1 N Etemadi etemadin@cc iut ac ir 2 C Ghobadi cyrus@cc iut ac ir Abstract Buttercup Ranunculus asiaticus L is a perennial and herbaceous plant belongs to the family of Ranunculaceae It is used as cut flower potted plant and bedding plant in landscapes The buttercup is an allogamus plant and its characters will be preserved through vegetative propagation Sexual propagation is the common method of multiplication of buttercup This is usually done with the use of F1 hybrid seeds in Iran Using micropropagation and tissue culture techniques can be the efficient methods for rapid multiplication and mass production of the buttercup plants Therefore in this research the effects of different explants including leaf axillary bud cotyledonary leaf and thalamus besides various levels of auxin and cytokinins on in vitro cultivation of buttercup plant Ranunculus asiaticus magic were investigated For regenrating all explants MS medium supplemented with different concentrations of 2 4 D BAP and kinetin were used The concentrations of plant growth regulators used in the experimants were as follows BAP 0 9 13 17 m and 2 4 D 0 2 2 4 4 8 8 m for cotyledonary leaf BAP 0 1 1 2 2 3 3 4 4 5 5 m and Kin 7 3 9 3 11 3 m for axillary bud BAP 0 2 2 4 4 8 8 m and 2 4 D 0 2 2 m for leaf BAP 0 2 2 4 4 6 6 m and 2 4 D 0 45 0 95 1 45 m for thalamus All treatments were arranged in the factorial experiments in a completely randomized design with three replications Statistical analysis was performed with SAS software and the Least Significant difference LSD method used for comparing treatment group means All explants other than thalamus could produce plantlets in cultures Results concerning the culture of explants in vitro indicated that the cotyledonary leaf explants cultured in MS medium supplemented with 2 2 m 2 4 D and 13 m BAP was proliferated more and produced the highest number of shoots The leaf explant had the highest regenerations and plantlet formation in MS medium containg 2 2 m BAP The best medium for producing maximum axillary buds from apical buds was MS medium supplemented with 11 3 m kin and 4 4 m BAP The recommended medium for induction establishment growth and direct organogenesis in thalamus was MS medium supplemented with 6 6 m BAP and 1 45 m 2 4 D In conclusion the result of the statistical analysis and the comparison of treatments group means indicated that the most efficient explant was axillary bud and the best culture medium was MS 11 3 m kin 4 4 m BAP The shoots were produced from all kinds of explants were rooted easily at 95 in hormone free MS medium Key words Ranunculus asiaticus Micropropagation Cotyledonary leaf Thalamus Axillary bud

نعمت ا... اعتمادي، سيروس قبادي

بدرالدين ابراهيم سيد طباطبائي

بهرام باني نسب، مجيد طالبي