عنوان :
تاثير محيط انجماد اسپرم بر توانايي لقاح و تكوين رويان هاي آزمايشگاهي گوسفند
مقطع تحصيلي :
كارشناسي ارشد
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
ده،97ص.:مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
نفيسه نيلي،محمد حسين نصر اصفهاني
استاد مشاور :
حميدرضا رحماني، محسن فروزان فر
توصيفگر ها :
لسيتين سويا , لقاح آزمايشگاهي
تاريخ نمايه سازي :
7/4/89
استاد داور :
محمد علي ادريس ،محمد فضيلتي
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Effect of Sperm Freezing Medium on Sperm Fertilizing Ability and Embryo Development in Ovine Mohsen Sharafi m sharafi@ag iut ac ir March 2010 Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language FarsiAbstractDuring the semen cryopreservation various biochemical and anatomical compartmentsin the sperm cells acrosome nucleus mithochondria axoneme and plasma membrane may be altered Therfore the first aim of sperm freezing protocols is use ofcryoprotectant agents in freezing medium in order to prevent lethal intracellular icecrystal formation and to reduce membrane damage during freezing thawing Althoughegg yolk is a major beneficial cryoprotectant for sperm freezing in recent years therehave been frequent arguments against the use of egg yolk one of which is the widevariability of composition that makes it difficult to analyze the beneficial effects of aparticular compound on sperm cryopreservation Furthermore egg yolk introduces arisk of microbial contamination with the subsequent production of endotoxins capableof damaging the fertilizing capacity of spermatozoa Regard these facts Substitution ofegg yolk with soybean lecithin may reduce these risks in freezing medium Though afew studies have been performed on the effect of soybean lecithin in bull to dateevaluation of ram semen in vitro fertility after cryopreservation with use of soybeanlecithin has not been studied This study assessed the effect of 1 or 2 wt vol soybean lecithin L1 or L2 or 15 or 20 vol vol egg yolk E15 or E20 supplemented with 5 or 7 glycerol G5 or G7 in a Tris based medium forcryopreservation of ram Oviss arries semen Semen samples were diluted withextenders and then frozen The sperm parameters were assessed after thawing formotility viability and acrosomal status In vitro Fertility was recorded as cleavage blastocyst and hatching rate The analysis of variance ANOVA was used forcomparisons of means The mean of the treatments were compared using Duncan smultiple range test DMRT and a confidence level of P 0 05 was considered to besignificant Although no significant difference was observed in pattern of acrosomalstatus the best results in terms of sperm motility viability and cleavage rates wereobserved with L1G7 51 9 4 8 48 1 3 5 and 79 6 3 9 respectively and E20G7 51 8 3 8 46 7 2 9 and 72 9 6 4 respectively Our results also showedthat 1 lecithin and 20 egg yolk was superior to 2 lecithin and 15 egg yolk Interms of cleavage rate 7 glycerol was superior to 5 glycerol No significantdifference was obtained between groups in terms of blastocysts rate per cleaved embryoand hatching rate Therefore we concluded that the optimal concentration of lecithinand egg yolk is 1 and 20 respectively along with 7 glycerol In addition ourresults suggest that lecithin can be used as a substitute for egg yolk KeywordsCryopreservation Freezing medium Soybean lecithin Sperm parameters IVF
استاد راهنما :
نفيسه نيلي،محمد حسين نصر اصفهاني
استاد مشاور :
حميدرضا رحماني، محسن فروزان فر
استاد داور :
محمد علي ادريس ،محمد فضيلتي
