5455

5115

كارشناسي ارشد

مهندسي شيمي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده مهندسي شيمي

1389

دوازده، 119ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

محمدرضا احساني، صبيحه سليمانيان زاد

كيخسرو كريمي

13/7/89

حميد زيلويي، مرتضي خان احمدي

مهندسي شيمي

ID5115

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

120Biological Production of Acetone Butanol and Ethanol from Rice Straw by Clostridium acetobutylicum Bacteria Farzad Moradi Gharibvand fm moradi@ce iut ac ir Date of Submission Apr 21 2010 Chemical Engineering Department Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language FarsiSupervisor M R Ehsani ehsanimr@cc iut ac irSupervisor S Soleimanianzad soleiman@cc iut ac irAbstractIncreasing in gasoline prices and depletion of fossil fuels in 80 s decade resulted inresearch and commercialization activities directed toward production of renewable fuelssuch as ethanol and butanol Renewable resources such as rice straw has been promotedas potential feedstock for production of fuels In this project production of Acetone Butanol and Ethanol ABE from rice straw as substrate by biological method wasinvestigated Rice straw is a lignocellulosic material which should convert to fermentablesugers At the first step the ability of bacterial fermentation on glucose synthetic mediumwith P2 stock solutions Buffer phosphate Vitamins and Minerals or PGY P2 solutionand PGY medium without P2 stock solutions was investigated After this stage andproof of ability of bacterial fermentationon on synthetic medium rice straw pretreated bythree different pretreatment methods and then hydrolyzed to sugers by enzymatichydrolysis Three different methods were used including dilute sulfuric acidpretreatment concentrated phosphoric acid pretreatment at modest reaction conditions 50 C and alkaline pretreatment by NaOH Then the pretreated rice straw washydrolyzed by enzymes These prepared hydrolyzates were fermented by clostridiumacetobutylicum bacteria In the fermentation step maximum theoretical solvent yield wasachieved 38 60 maximum yield on substrate drived from alkaline pretreatment enzymatic hydrolysis and 37 99 for substrate drived from phosphoric acid pretreatment enzymatic hydrolysis This amount for dilute acid pretreatment enzymatic hydrolysissubstrate was very low only 3 44 The above results showed if appropriatepretreatment methods used for rice straw this lignocellulosic biomass would be a suitablesubstrate for this fermentation process Keywords Acetone Butanol Ethanol ABE Rice straw C acetobutylicum Pretreatment Enzymatic hydrolysis Fermentation

محمدرضا احساني، صبيحه سليمانيان زاد

كيخسرو كريمي

حميد زيلويي، مرتضي خان احمدي