5468

5128

كارشناسي ارشد

پليمر

اصفهان: دانشگاه صنعتي اصفهان، دانشكده مهندسي شيمي

1389

پانزده،88ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

مرتضي صادقي، سعيد كرباسي

سعيد نوري خراساني

24/7/89

محمود معصومي، محمد رفيعي نيا

مهندسي شيمي

ID5128

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

122 Degradation Mechanism and Morphology of Poly hydroxybutyrat nanohydroxyapetite PHB nHA Nanocomposite Scaffold Used in Tissue Engineering Mohsen Radmehr mm Radmehr@ce iut ac ir Date of Submission 2010 05 11 Department of Chemical Engineering Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisors M Sadeghi m sadeghi@cc iut ac ir S Karbasi karbasi@med mui ac irAbstract Nowadays biodegradable polymers bioactive ceramics composites with good physicaland mechanical properties and biocompatibility are one of important subjects for researchers inbone tissue engineering This study is focused on fabrication of nanocomposite scaffold ofPoly 3 hydroxybutyrate nanohydroxyapetite PHB nHA via solvent casting and particulateleaching technique and study of morphology and degradation mechanism of them Therefore scaffold samples have been prepared with PHB chloroform solution with the PHB NaCl weightratio of 10 90 15 85 and 20 80 Amount of nHA has been varied for each series and it has been inrange of 0 10 wt belong to PHB Porosity percentage of scaffolds has been calculated usingdeionized water diffusion method Interaction between two phases of nanocomposites has beenstudied by using Furrier Transform Inferred FTIR Morphology and distribution of porosity morphology and size of nHA and distribution of nHA in PHB matrix of scaffolds have beenexamined by using Scanning Electron Microscopy SEM and Energy Dispersive X ray EDX Itseems some hydrogen bonding interaction between carbonyl group of PHB and hydroxyl group andPO43 of nHA These reasons indicate the good interaction between nHA and PHB in the interfaceof nanocomposite for controlling size and morphology of nano particles The SEM results haveshown the porosity size in the range of 200 250 m The interconnectivity of scaffold has beenshown by the existing cavities on the wall of porosity for prepared scaffold with 90wt NaCl Decrease of porosity percentage of samples by increasing nHA is not significant Degradation ofnanocomposite scaffolds in Phosphate Buffer solution PBS T 38 2 C has been studied bymeasuring weigh of samples and measurement of PBS pH for 102 days SEM and FTIR analysishave been used for study of scaffold morphology and product of scaffold degradation after andbefore degradation Weigh measurement of samples measurement of PBS pH and SEM imagehave illustrated that degradation of PHB nHA scaffold is bulk degradation Maximum degradationhas been seen for prepared scaffold with 90wt NaCl and 7 5wt nHA Generally if mechanicalproperties are not seen and according to results of all analysis prepared scaffold with 90wt NaCland 7 5wt nHA is preferred for growing bone cells on it The obtained results suggest thesenewly developed PHB nHA composite scaffolds may serve as a three dimensional substrate inbone tissue engineering Keywords hydroxyapetite nanocomposite scaffold poly hydroxybutyrate solvent castingand particulate leaching bone tissue engineering

مرتضي صادقي، سعيد كرباسي

سعيد نوري خراساني

محمود معصومي، محمد رفيعي نيا