5669

5291

كارشناسي ارشد

علوم دامي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1389

دوازده،73ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

محمد علي ادريس

رحماني، احمد خوروش

11/12/89

انصاري، طباطبائي

مهندسي كشاورزي

ID5291

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Relation between FXI and DGAT1 Genes with Reproductive Traits in Cows Holstein Neda Naghdi n naghdi@ag iut ac ir October 2 2010 Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Prof Mohhamad Ali Edriss Email edriss@cc iut ac ir Abstract In dairy farms augmentation of reproduction s efficiency is essential for economical milk production Total milk production of a cow mostly depends on number of milking period times amount of milk production per period Many factors such as genetic physiologic and environmental factors and manner of farm management can affect on pregnancy rate However finding of the main reasons for low reproduction s efficiency are very difficult due to many complicated factors affecting on Among important factors affecting reproduction rate are factor XI FXI and Diacylglycerol acyltransferase DGAT1 Factor XI is a blood coagulation factor and deficiency of this factor may decrease growth of ovarian follicle A QTL has been mapped consistently to the centromeric region of the bovine chromosome 14 that appears to have a main positive effect for variation in fat content of milk The DGAT1 encodes for acylCoA diacylglycerol acyltransferase 1 an enzyme that catalyses the final step of triglyceride synthesis It was proposed to be a strong candidate gene underlying this effect due both to its appropriate chromosomal location and the fact that knock out transgenic mice lacking both functional alleles of DGAT1 were totally devoid of lactation The objective of this study was to determine FXI and DGAT1 genes polymorphism and the relationship with Holstein dairy cow s reproduction traits Blood samples were collected from 120 Holstein cows of four different herd management in Isfahan region 94 cows had repeat breeding failure and 26 cows were randomly selected Isolation of genomic DNA was performed using a salting out method For FXI gene no mutant alleles have been identified and all of these samples showed a single 244bp band The polymerase chain reaction was used to amplify the 378bp DNA fragments from genomic DNA for DGAT1 gene PCR products were digested with BglI enzyme At the DNA level it is caused by double substitution at the nucleotide position 10433 and 10434 in exon 8 alanine instead of lysine lysine encoding allele K was visible in gel as two bands of 282bp and 96bp Alanine encoding allele A was cleaved into three fragments of 254bp 96bp and 28bp The frequency of AA AK and KK genotypes was 0 59 0 34 and 0 06 respectively The K and A alleles frequencies were 0 23 and 0 77 respectively in Holstein cattle Mixed model analysis was used to estimate the effect of the DGAT1 K232A polymorphism for milk production and reproductive traits The K232A polymorphism was significantly associated with percentage of milk and length of pregnancy and not shown to be significantly associated with any other traits Key Word Factore XI Diacylglycerol acyltransferase Polymerase Chain Reaction Restriction enzyme

محمد علي ادريس

رحماني، احمد خوروش

انصاري، طباطبائي