5774

5389

كارشناسي ارشد

شناسايي و انتخاب مواد

اصفهان: دانشگاه صنعتي اصفهان، دانشكده مواد

1389

چهارده،110ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

محمد حسين فتحي،محمدعلي گلعذار

علي مهدي كوچكسرايي، شيوا مرتضوي

29/1/90

فخرالدين اشرفي زاده،سعيد نوري خراساني

مهندسي مواد

ID5389

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Design and Preparation of a Biodegradable Scaffold Based on Poly Caprolactone and Nano Powder Flouradated hydroxyapatite for Bone Tissue Engineering Narges Johari n johari@ma iut ac ir Date of Submission 7 March 2011 Department of Materials Engineering Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisors Dr Mohammad Hossein Fathi fathi@cc iut ac ir Dr Mohammad Ali Golozar golozar@cc iut ac ir Abstract In this study poly caprolactone nano fluoridated hydroxyapatite PCL FHA scaffold were produced Fluoridated hydroxyapatite FHA nanopowders had a chemical composition of Ca10 PO 4 6OH2 xFx where x values were selected equal to 0 5 1 1 5 and 2 0 Different weight fractions of 10 20 30 and 40 of the FHA were added to the PCL PCL FHA scaffolds were prepared by solvent casting particulate leaching method Sodium chloride NaCl particle was used as a porogen with diameters 250 400 m Structural chemical mechanical bioactivity biodegradation and biocompatibility properties of scaffolds were evaluated The pore morphology size and distribution of the scaffolds were characterized using a scanning electron microscope SEM X ray diffraction XRD and Fourier transform infrared spectroscopy FTIR were used to determine the phase structure chemical composition and functional groups of scaffolds Porosity of the scaffolds was measured using Archimedes Principle according to a known method These scaffolds were evaluated for their compressive mechanical properties The bioactivity of the nanocomposite scaffolds was evaluated by soaking the scaffolds in a simulated body fluid SBF up to 4 week The changes of pH of the SBF medium were measured at pre determined time intervals using a pH meter The calcium and phosphorous ions concentrations of SBF medium were determined using ICP OES analysis The apatite formation on the surface of the scaffolds as consequence of the dissolution and precipitation process of calcium phosphate was investigated by scanning electron microscopy SEM In vitro degradation was studied by immersing of scaffolds in phosphate buffered saline PBS up to 30 days The changes of pH of the PBS and the changes of the weight loss of the scaffolds were measured MTT test carried out to investigate the cytotoxicity cell proliferation and cell attachment Results showed a uniform scaffold relatively that by increasing of NaCl amount its porosity was increased Compressive strength bioactivity and biodegradation of scaffolds increased with the increasing the weight ratio of FHA Compressive strength also decreased with the increasing of the porosity and the fluorine content of FHA However biodegradation of scaffolds increased by increasing of the fluorine content of FHA The increase in ionic concentration of calcium and phosphorus as well as the increase in pH after SBF immersion resulted in an increase in ionic activity product of apatite in the solution The SEM micrographs of scaffolds after immersing in SBF showed that the PCL FHA nanocomposite scaffolds can induce a dense and continuous layer of apatite after soaking for 4 weeks The G292 osteoblast cell culture showed the cytotoxicity and cell attachment of scaffolds On the other hand the MTT test showed that cell proliferation was increased when the porosity of scaffolds was increased Keywords Scaffold Poly caprolactone Nano fluoridated hydroxyapatite Solvent casting particulate leaching method Bioactivity Biocompatibility Biodegradation Compressive strength

محمد حسين فتحي،محمدعلي گلعذار

علي مهدي كوچكسرايي، شيوا مرتضوي

فخرالدين اشرفي زاده،سعيد نوري خراساني