5877

5492

كارشناسي ارشد

بيوتكنولوژي كشاورزي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1389

يازده،58ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

سيروس قبادي، بدرالدين ابراهيم سيد طباطبائي

ابراهيم گنجي مقدم

14/2/90

علي اكبر رامين، مجيد طالبي

مهندسي كشاورزي

ID5492

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Genetic variabiling among sixty cherry genotypes Prunus mahaleb used as rootstock revealed by SSR and ISSR marker Hamidreza Golmohammadi h golmohammady@gmail com Date of submission February 22 2011 Department of Agricultural Biotechnology Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi 1 S Ghobadi Assoc W Supervisor cyrus@cc iut ac ir d W Supervisor Sayed @cc iut ac ir Abstract Sweet cherry Prunus avium L Rosaceae 2n 16 is cultivated in temperate regions of the world for its edible fruit Iran is one of the three biggest cherry producer after US and Turkey in the world Annual production of all kinds of cherry in Iran exceeded 200000 tons FAO In recent years a great research has been conducted on sweet cherry and sour cherry breeding specially on their rootstocks with aims to introduce dwarf and compatible ones All commercially grown sweet cherries use a rootstock in combination with a grafted or budded scion variety Rootstocks can directly influence productivity precocity tree size tree architecture fruit size and fruit quality The choice of certain rootstocks will also influence many horticultural decisions such as pruning training tree support and labor management Mazard Prunus avium Colt Sour cherry Prunus cerasus and Mahaleb Prunus mahaleb are used as a common rootstock for sweet cherry in the world The best cherry rootstock for Iranian nurseries and orchards is Prunus mahaleb Mahaleb has been known as a cultivar with strong roots which grows as well as tolerant plants in poor and dry soil such as soil with iron and zinc deficiency Mahaleb demonstrates a cold tolerance trait and can endure temperatures as low as 20 C so it can be a suitable rootstock for climate of Iran However Iran has a wide spectrum of Mahaleb s genetic diversity but the phylogenic information of Iranian accession are not available Therefore this study has been done with aims to evaluate genetic variation of Mahaleb germplasms which collected at the Horticultural Department of Mashhad Iran Owing to have a little special primers of this plant In this study genetic variation of 60 Mahaleb genotypes was assessed by using 9 pairs of sweet cherry 4 pairs sour cherry and two pairs of peach SSR primers Ten out of fifteen primers demonstrated two to five alleles with an average of 2 11 alleles per primer and it showed 0 4 heterozygosity and average of polymorphism information content PIC was 0 46 The SSR markers dendrogram has been illustrated by power marker software with coefficient Nei 1983 and the Neighbor joining method Analysis of SSR data clustered the mahaleb genotypes into six group The third group has 15 genotypes The 7 of 15 accessions were dwarf and late flowered Thirteen out of thirty one ISSR markers have been tested and produced 40 polymorphic band ISSR data were used to illustrate dendrogram in NTSYS V Pc 2 02e by using Jaccards similarity coefficient and Neighbor joining method The result demonstrate that Mahaleb genotypes have been divided into seven groups ISSR s demdrogram in contrast with SSR s dendrogram showed no relation between morphological characters The result demonstrate that ISSR markers are hardly able to show genetic variations between Mahaleb accessions Key Words Sweet cherry rootstock Dwarf rootstock Mahaleb ISSR

سيروس قبادي، بدرالدين ابراهيم سيد طباطبائي

ابراهيم گنجي مقدم

علي اكبر رامين، مجيد طالبي