5879

5494

كارشناسي ارشد

بيوتكنولوژي كشاورزي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1389

سيزده،98ص.: مصور﴿بخش رنگي﴾،جدول،نمودار

ص.ع.به فارسي و انگليسي

بدرالدين ابراهيم سيد طباطبائي

14/2/90

نعمت ا... اعتمادي، آقا فخر مير لوحي

مهندسي كشاورزي

ID5494

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Detection of Somaclonal Variation in Micro propagated Capsicum annuum using SRAP Marker Zohre Moheb Mohammadi zohremohebmohamadi@yahoo com Date of submission March 6 2011 Department of Agricultural Biotechnology Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi 1 B E Seyed Tabatabaei Professor Supervisor Sayedt @cc iut ac ir 2 M Talebi Ph D Assist Professor Superivisor mtalebi@cc iut ac ir Abstract Pepper Capsicum annuum is a vegetable with vitamins and minerals which being used as a drug as well as nutritional usage The plant could be propagated from hybrid seeds Tissue culture technique could rapidly propagate this plant with a homogeneous genetic structure in a large scale The application of modern biotechnology for improvement of pepper productivity requires an efficient in vitro plant regeneration protocol An attempt was made in this study to evaluate the effectiveness of different genotypes 12 genotypes of bell peppers two sweet peppers and one ornamental pepper growth regulators and explants leaves and cotyledons on callus production and regeneration and introduce appropriate protocol for micropropagation of a commercial variety of pepper in Iran Analysis of variance indicated that the effectiveness of different genotypes interaction of various explants and combination of hormones and interaction between genotypes explants and hormones were significant on the evaluated traits at 1 level Therefore in micropropagation protocols different genotypes explants and compound of hormones should be considered The results obtained in this research confirmed the synergistic effect of the combination of BAP with IAA in the regeneration of adventitious shoots previously observed by other authors Among the 15 varieties tested Lirica 35 906 RZ Orange Max Zamboni Olite and Ganga were given the best shoot differentiation response average over 14 shoots per explant from the leaf explants In summary for micropropagation of pepper with cotyledons and leaves combination of 5 mg l BAP and 1 mg l IAA was selected Elongation of regenerated shoots has been promoted with gibberellic acid GA3 1 mg l supplemented medium In this study at least 80 of the plants regenerated in vitro survived the acclimation period Somaclonal variation reduced the commercial value of plants Thus it is very important to detect somaclonal variation at earlier stage of plant growth to avoid economic loss The basic objective of micropropagation is to produce the true to type plants therefore it is important to certify and retain the fidelity of produced regenerants Considering high efficiency of leaf explants regeneration 48 leaf regenerated plants in 5 genotypes according to their regeneration yield and appearance were selected for genetic stability evaluation Somaclonal

بدرالدين ابراهيم سيد طباطبائي

نعمت ا... اعتمادي، آقا فخر مير لوحي