تنوع جمعيتي سيچليد ايراني Iranocichla hormuzensis رودخانه مهران و سرچشمه خورگو استان هرمزگان

چكيده انگليسي :

Population Diversity of Iranian Cichlid Iranocichla hormuzensisFrom Mehran River and Khorgo Spring in Hormuzgan Province Zahra Ghasabshiran Zahra ghasabshiran@yahoo com April 6 2011 Department of Natural Resources Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiDr Salar Dorafshan sdorafshan@cc iut ac irAbstractThe Iranian cichlid Iranocichla hormuzensis the only member of cichlidae family in Iran is widely distributedin Hormuz basin rivers such as Mehran and Khorgo Spring Considering the importance of the genetic variationand population structure for management and conservation of species especially native fish the genetic diversityof Iranian cichlid was investigated using morphological and molecular techniques In this study 30 specimens ofIranian cichlid were collected from each of the two locations Mehran river and Khorgo Spring Morphologicalinvestigation included 13 truss land marks and 13 meristic traits For the analysis of data resulted from land marks and meristic traits principal component analysis and non parametric U Manwithni test were used respectively Two populations could be separated based on morphological analysis Significant differencesamong nine land marks and four meristic traits were observed t test p 0 05 For molecular study DNA wasextracted using ammonium acetate method from fin clips and or apart of bodies 30 samples from each stations The DNA quality was examined by electrophoresis on 1 agaros gel and painted with ethidium bromide PCRamplification was performed using four pairs of microsatellite primers OS7R OS25 OS7 and OS64 atannealing temperature 53 59 9 PCR products electrophoresed on 12 achrylamid gel and painted with silvernitrate Calculations was done using GenAlex 6 software All loci were polymorphic Number of the observedalleles at all loci were in the range of 4 9 Average number of observed alleles in Khorgo spring and Mehranriver were 5 and 5 75 respectively The number of effective alleles at all loci was in the range of 1 90 5 84 Average number of effective alleles in Khorgo Spring and Mehran River were 3 96 and 3 63 respectively Allelsizes at OS7R OS25 OS7 OS64 loci were in the range of 78 94 76 96 104 150 38 68 bps respectively Themean of observed heterozygosity in Khorgo Spring and Mehran River were 0 78 and 0 60 respectively Themean of expected heterozygosity in Khorgo Spring and Mehran River were 0 71 and 0 68 respectively Resutsshowed deviation from Hardy Weinberg equilibrium at five OS7R OS25 and OS64 loci in Khorgo Spring andOS7R and OS64 loci in Mehran river out of eight studied loci Increase of observed heterozygosity towardsexpected heterozygosity at OS7 OS25 and OS64 loci in Khorgo spring and OS7 locus in Mehran river low 3 genetic segregation between population high 97 genetic diversity within population and high 10 76 noticable gene flow were colculated Genetic identity and genetic distance between two populations were 0 88and 0 12 respectively Genetic diversity in Khorgo spring was higher 0 78 than Mehran river 0 60 The lowgenetic distances between two populations maybe caused by same ancestor of two populations Althoughmorphological analysis showed significant differences between the two populations microsatellite techniquecould not segregate them However molecular technique showed desirable genetic diversity in each population It is probably because of using non specific primers and low number of samples For better understanding ofpopulation structure of Iranian cichlid as a native fish species of Iran population structure studies using othermolecular techniques from all distributed areas followed by ecological and or biological researches should beconsidered Key Words Iranian cichlid Iranocichla hormuzensis morphology microsatellite genetic diversity Hormozgan

قصاب شيران، زهرا

تنوع جمعيتي سيچليد ايراني Iranocichla hormuzensis رودخانه مهران و سرچشمه خورگو استان هرمزگان

مورفولوژي , تنوع زنتيكي , ريز ماهواره